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| Status |
Public on Mar 07, 2022 |
| Title |
Next-generation sequencing of CUT&RUN of MKL-1 Merkel cell carcinoma cells treated with EPZ011989 |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The overarching goal of this study was to characterize mechanisms of sensitivity to EZH2 inhibitors in Merkel cell carcinoma cell lines. To that end, CUT&RUN for H3K27me3 and H3K4me3 histone marks was performed in MKL-1 cells treated with the EZH2 inhibitor EPZ011989 for 6 days.
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| Overall design |
MKL-1 cells were treated in duplicate with 3 µM EPZ011989 or vehicle (1% ethanol) for 6 days with refreshment on day 3. Each sample was split into thirds and CUT&RUN was performed with antibodies against H3K27me3 or H3K4me3 or control IgG. 12 samples are analyzed.
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| |
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| Contributor(s) |
Gartin AK |
| Citation(s) |
35331717 |
| |
| Submission date |
Nov 01, 2021 |
| Last update date |
Jun 13, 2022 |
| Contact name |
Ashley K. Gartin |
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Lab |
DeCaprio Lab
|
| Street address |
450 Brookline Ave. Mayer 444
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE186899 |
MKL-1 Merkel cell carcinoma cells treated with EPZ011989 |
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| Relations |
| BioProject |
PRJNA776721 |
| SRA |
SRP343994 |
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