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| Status |
Public on Oct 30, 2021 |
| Title |
The Role of Sorting Nexin 17 in Cardiac Development |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Homozygous deletion of the SNX17 gene in rats resulted in mid-gestational embryonic lethality which was accompanied by congenital heart defects, including in the double-outlet right ventricle, atrioventricular and ventricular septal defects. To elucidate the potential mechanisms underlying development of cardiac OFT defects induced by SNX17 knockout, we performed RNA-seq analysis in cardiac outflow tract (OFT) tissues isolated from WT and HO embryos at E13.5. A total of 262 differentially expressed genes (DEGs) were identified between WT and HO samples, of which 94 and 168 were up-regulated and down-regulated, respectively. Analysis of biological functions of the DEGs, via Gene Ontology (GO), revealed that the up-regulated genes were mainly involved in heart development, and negative regulation of the intrinsic apoptotic pathway, while the down-regulated DEGs were associated with cell adhesion, extracellular organization, and negative regulation of the Wnt signaling pathway.
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| Overall design |
RNA-seq analysis in cardiac OFT tissues isolated from WT and HO embryos at E13.5.
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| Contributor(s) |
Wu Y, Zhou Y, Huang J, Ma K, Yuan T, Jiang Y, Ye M, Li J |
| Citation(s) |
34988124 |
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| Submission date |
Oct 27, 2021 |
| Last update date |
Jan 11, 2022 |
| Contact name |
Yufei Wu |
| Organization name |
School of Medicine, Tongji University
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| Street address |
Siping Campus,1239 Siping Road, Yangpu District, Shangha.
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL19482 |
Ion Torrent Proton (Rattus norvegicus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA775617 |
| SRA |
SRP343453 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE186706_E13.5_OFT_all.counts.xls.gz |
1.9 Mb |
(ftp)(http) |
XLS |
| GSE186706_E13.5_OFT_all.rpkm.xls.gz |
2.2 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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