|
| Status |
Public on Jun 15, 2022 |
| Title |
Determination of human DNA replication origin position and efficiency reveals principles of initiation zone organisation |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We describe a modification of initiation site sequencing (ini-seq, Langley et al., NAR 2016) in which density gradient centrifugation separates ‘heavy’ replicated DNA containing BrdU from DNA containing only ‘light’ dTTP before sequencing. This approach allows us to assign a replication initiation efficiency score to each of the 23905 origins identified. We also performed Short Nascent Strand sequencing (SNS-seq), an established method to map replication origins, in the same cell line as in Ini-seq2 for comparison.
|
| |
|
| Overall design |
Massive sequecing of human replication origin isolated from EJ30 cell line by either Ini-seq2 or SNS-seq method
|
| |
|
| Contributor(s) |
Guilbaud G, Murat P, Wilkins H, Lerner LK, Sale JE, Krude T |
| Citation(s) |
35801867 |
| |
| Submission date |
Oct 27, 2021 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Guillaume Guilbaud |
| E-mail(s) |
guilbaud@mrc-lmb.cam.ac.uk
|
| Organization name |
Medical Research Council - LMB
|
| Department |
PNAC
|
| Lab |
Julian E Sale
|
| Street address |
Francis Crick Avenue
|
| City |
Cambridge |
| ZIP/Postal code |
CB20QH |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA775057 |
| SRA |
SRP343426 |
Less...
More...