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| Status |
Public on Jun 30, 2022 |
| Title |
Functional Recovery from Human Pluripotent Stem-Derived Dopamine Neuron Grafts is Dependent on Neurite Outgrowth |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We conducted bulk RNAseq to understand changes in the transcriptome of in vitro dopaminergic progenitor cells as their duration of differentiation changes. We obtained gene expression estimates from Day 13, (n=2), Day 18 (n=2), and Day 25 (n=2) cell cultures.
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| Overall design |
Two Parkinson's Disease patients provided skin cells that were reprogrammed -> fibroblasts -> iPSC -> Dopaminergic neurons. At Days 13, 18 and 25 of dopeminergic neuron differentiation, cells were isolated for bulk RNA-seq. Paired-end RNA sequencing was performed on the Illumina HiSeq 2000 platform, with TruSeq_v2 library preparation chemistry.
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| Contributor(s) |
Mossman JA, Loring JF, Williams RM |
| Citation(s) |
37166357 |
| |
| Submission date |
Oct 20, 2021 |
| Last update date |
Jul 07, 2024 |
| Contact name |
Jeanne Loring |
| Organization name |
Scripps Research
|
| Department |
The Center for Regenerative Medicine
|
| Lab |
Loring Laboratory
|
| Street address |
10550 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| GSM5641609 |
Dopaminergic neuron precursor cells - HDF410 - Day 13 |
| GSM5641610 |
Dopaminergic neuron precursor cells - HDF411 - Day 13 |
| GSM5641611 |
Dopaminergic neuron precursor cells - HDF410 - Day 18 |
| GSM5641612 |
Dopaminergic neuron precursor cells - HDF411 - Day 18 |
| GSM5641613 |
Dopaminergic neuron precursor cells - HDF410 - Day 25 |
| GSM5641614 |
Dopaminergic neuron precursor cells - HDF411 - Day 25 |
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| Relations |
| BioProject |
PRJNA773064 |
| SRA |
SRP342317 |
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