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Series GSE186069 Query DataSets for GSE186069
Status Public on Mar 11, 2022
Title Systematic reconstruction of the cellular trajectories of mammalian embryogenesis (E8.5)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Mammalian embryogenesis is characterized by rapid cellular proliferation and diversification. Within a few weeks, a single cell zygote gives rise to millions of cells expressing a panoply of molecular programs, including much of the diversity that will subsequently be present in adult tissues. Although intensively studied, a comprehensive delineation of the major cellular trajectories that comprise mammalian development in vivo remains elusive.
For mouse embryogenesis in particular, we and others have performed single cell or single nucleus RNA-seq data (scRNA-seq) during implantation, gastrulation and organogenesis. Here we set out to integrate several single cell RNA-seq datasets (scRNA-seq) that collectively span mouse gastrulation and organogenesis. However, a technical challenge that we faced is that the datasets that we sought to integrate were generated by different groups at different times using different scRNA-seq technologies. In particular, probably because there was no overlapping timepoint, the integration of scRNA-seq data generated at E8.5 (cells, 10X Genomics) and E9.5 (nuclei, sci-RNA-seq3) was challenging (Cao et al. 2019; Pijuan-Sala et al. 2019).
To address this, we set out to generate new data at E8.5 that might serve to “bridge” these two datasets. Because of how quickly changes are occurring during this window of development, we focused on individual, somite-resolved E8.5 embryos using a simplified, optimized version of sci-RNA-seq3. We selected 12 embryos from 2 separate litters harvested at E8.5, including a single primitive streak stage embryo (prior to somitogenesis) and 11 embryos staged in 1-somite increments from 2 to 12 somites. The optimized sci-RNA-seq3 method markedly improved data quality, with 9-fold higher UMIs and 6-fold higher gene detection per nucleus, relative to (Cao et al. 2019).
Overall, we collected published data (Cheng et al. 2019; Mohammed et al. 2017; Pijuan-Sala et al. 2019), the new E8.5 data, and published data from one study spanning E9.5 to E13.5 but with deeper sequencing of those libraries (Cao et al. 2019). Altogether, we define cell states at each of 19 successive stages spanning E3.5 to E13.5, heuristically connect them to their pseudo-ancestors and pseudo-descendants. Despite being constructed through automated procedures, the resulting trajectories of mammalian embryogenesis (TOME) are largely consistent with our contemporary understanding of mammalian development.
In addition, the new E8.5 data itself comprises a foundational resource for mammalian developmental biology (especially for the early somitogenesis), and are made available in a way that will facilitate their ongoing annotation by the research community.
 
Overall design Around 240 thousand cells derived from 12 individual, somite-resolved E8.5 mouse embryos. The 12 samples are from 2 separate litters harvested at E8.5, including a single primitive streak stage embryo (prior to somitogenesis) and 11 embryos staged in 1-somite increments from 2 to 12 somites.
 
Contributor(s) Qiu C, Cao J, Martin B, Li T, Welsh IC, Srivatsan S, Huang X, Calderon D, Noble WS, Disteche CM, Murray SA, Spielmann M, Moens CB, Trapnell C, Shendure J
Citation(s) 35288709, 37066300, 38355799
Submission date Oct 18, 2021
Last update date Mar 06, 2024
Contact name Chengxiang Qiu
E-mail(s) cxqiu@uw.edu
Organization name University of Washington
Department Genome Sciences
Lab Jay Shendure
Street address William H. Foege Hall, 3720 15th Ave NE
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (465)
GSM5630860 sci-RNA-seq3 with E8.5 mouse embryo P2-01A
GSM5630861 sci-RNA-seq3 with E8.5 mouse embryo P2-01B
GSM5630862 sci-RNA-seq3 with E8.5 mouse embryo P2-01C
This SubSeries is part of SuperSeries:
GSE186070 Systematic reconstruction of the cellular trajectories of mammalian embryogenesis
Relations
BioProject PRJNA772276
SRA SRP341947

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Supplementary file Size Download File type/resource
GSE186069_cell_annotate.csv.gz 4.3 Mb (ftp)(http) CSV
GSE186069_gene_annotate.csv.gz 943.7 Kb (ftp)(http) CSV
GSE186069_gene_count.mtx.gz 2.1 Gb (ftp)(http) MTX
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Raw data are available in SRA
Processed data are available on Series record
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