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GEO help: Mouse over screen elements for information. |
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Status |
Public on Oct 15, 2021 |
Title |
Screening for potential target genes of hsa-mir-199a-1 and hsa-mir-4423 using Clariom D arrays |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
We screened for target genes of hsa-mir-199a-1 and hsa-mir-4423 by transcriptome profiling. The miRNAs were overexpressed in HeLa cells by transient transfection of precursor miRNA expression plasmids. High-density Clariom D arrays for human were used for the gene expression analysis. This GEO entry provides the microarray data preprocessed according to the gene level workflow.
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Overall design |
HeLa cells were transiently transfected with non-viral precursor miRNA expression plasmids from GeneCopoeia (HmiR0296-MR04 and HmiR1083-MR04, respectively). As negative control, a plasmid with scrambled sequence (CmiR0001-MR04, GeneCopoeia) was used. Total RNA was isolated 24 h (n=2 replicates) and 48 h (n=4 replicates) following transfection. For all 18 RNA samples, transcript levels were measured with Clariom D arrays.
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Contributor(s) |
Hecker M, Koczan D, Boxberger N, Zettl UK |
Citation(s) |
35561450 |
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Submission date |
Oct 13, 2021 |
Last update date |
May 20, 2022 |
Contact name |
Michael Hecker |
E-mail(s) |
michael.hecker@rocketmail.com
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Organization name |
University of Rostock
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Department |
Department of Neurology
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Lab |
Division of Neuroimmunology
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Street address |
Gehlsheimer Str. 20
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City |
Rostock |
ZIP/Postal code |
18147 |
Country |
Germany |
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Platforms (1) |
GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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Samples (18)
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GSM5624842 |
negative control, 24 h post-transfection, replicate 1 |
GSM5624843 |
negative control, 24 h post-transfection, replicate 2 |
GSM5624844 |
negative control, 48 h post-transfection, replicate 1 |
GSM5624845 |
negative control, 48 h post-transfection, replicate 2 |
GSM5624846 |
negative control, 48 h post-transfection, replicate 3 |
GSM5624847 |
negative control, 48 h post-transfection, replicate 4 |
GSM5624848 |
mir-199a-1 overexpression, 24 h post-transfection, replicate 1 |
GSM5624849 |
mir-199a-1 overexpression, 24 h post-transfection, replicate 2 |
GSM5624850 |
mir-199a-1 overexpression, 48 h post-transfection, replicate 1 |
GSM5624851 |
mir-199a-1 overexpression, 48 h post-transfection, replicate 2 |
GSM5624852 |
mir-199a-1 overexpression, 48 h post-transfection, replicate 3 |
GSM5624853 |
mir-199a-1 overexpression, 48 h post-transfection, replicate 4 |
GSM5624854 |
mir-4423 overexpression, 24 h post-transfection, replicate 1 |
GSM5624855 |
mir-4423 overexpression, 24 h post-transfection, replicate 2 |
GSM5624856 |
mir-4423 overexpression, 48 h post-transfection, replicate 1 |
GSM5624857 |
mir-4423 overexpression, 48 h post-transfection, replicate 2 |
GSM5624858 |
mir-4423 overexpression, 48 h post-transfection, replicate 3 |
GSM5624859 |
mir-4423 overexpression, 48 h post-transfection, replicate 4 |
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Relations |
BioProject |
PRJNA771013 |
Supplementary file |
Size |
Download |
File type/resource |
GSE185859_RAW.tar |
367.9 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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