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| Status |
Public on Oct 04, 2021 |
| Title |
Interaction between maternal immune activation and peripubertal stress in rats: impact on cocaine addiction-like behaviour, morphofunctional brain parameters and striatal transcriptome |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Substance use disorders are more prevalent in schizophrenia, but the causal links between both conditions remain unclear. Maternal immune activation (MIA) is associated with schizophrenia which may be triggered by stressful experiences during adolescence. Therefore, we used a double-hit rat model, combining MIA and peripubertal stress (PUS), to study cocaine addiction and the underlying neurobehavioural alterations. We injected lipopolysaccharide or saline on gestational days 15 and 16 to Sprague-Dawley dams. Their male offspring underwent five episodes of unpredictable stress every other day from postnatal day 28 to 38. When animals reached adulthood, we studied cocaine addiction-like behaviour, impulsivity, Pavlovian and instrumental conditioning, and several aspects of brain structure and function by MRI, PET and RNAseq. MIA facilitated the acquisition of cocaine self-administration and increased the motivation for the drug; however, PUS reduced cocaine intake, an effect that was reversed in MIA + PUS rats. We found concomitant brain alterations: MIA + PUS altered the structure and function of the dorsal striatum, increasing its volume and interfering with glutamatergic dynamics (PUS decreased the levels of NAA + NAAG but only in LPS animals) and modulated specific genes that could account for the restoration of cocaine intake such as the pentraxin family. On its own, PUS reduced hippocampal volume and hyperactivated the dorsal subiculum, also having a profound effect on the dorsal striatal transcriptome. However, these effects were obliterated when PUS occurred in animals with MIA experience. Our results describe an unprecedented interplay between MIA and stress on neurodevelopment and the susceptibility to cocaine addiction.
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| Overall design |
Gene expression profiles of the nucleus accumbens and dorsolateral striatum of male Sprague-Dawley rats after prenatal immune activation induced by LPS administration and peripubertal unpredictable stress. We analysed 24 samples (3 per group) corresponding to experimental groups (including saline-exposed and/or non-stressed controls).
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| Contributor(s) |
Capellán R, Higuera-Matas A |
| Citation(s) |
36890154 |
| |
| Submission date |
Oct 02, 2021 |
| Last update date |
Jul 19, 2024 |
| Contact name |
Alejandro Higuera-Matas |
| E-mail(s) |
ahiguera@psi.uned.es
|
| Phone |
913989689
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| Organization name |
Universidad Nacional de Educación a Distancia (UNED)
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| Department |
Psychobiology
|
| Street address |
Calle Juan del Rosal 10
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA768096 |
| SRA |
SRP339774 |
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