 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 10, 2023 |
| Title |
Highly concentrated trehalose induces prohealing senescence-like state in fibroblasts via CDKN1A/p21 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Trehalose is the nonreducing disaccharide of glucose, evolutionarily conserved in invertebrates. The living skin equivalent (LSE) is an organotypic coculture containing keratinocytes cultivated on fibroblast-populated dermal substitutes. We demonstrated that human primary fibroblasts treated with highly concentrated trehalose promote significantly extensive spread of the epidermal layer of LSE without any deleterious effects. The RNA-seq analysis of trehalose-treated 2D and 3D fibroblasts at early time points revealed the involvement of the CDKN1A pathway, the knockdown of which significantly suppressed the upregulation of DPT, ANGPT2, VEGFA, EREG, and FGF2. The trehalose-treated fibroblasts were positive for senescence-associated β-galactosidase. Finally, transplantation of the dermal substitute with trehalose-treated fibroblasts accelerated wound closure and increased capillary formation significantly in the experimental mouse wounds in vivo, which was canceled by the CDKN1A knockdown. These data indicate that high-concentration trehalose can induce the senescence-like state in fibroblasts via CDKN1A/p21, which may be therapeutically useful for optimal wound repair.
|
| |
|
| Overall design |
RNA-seq analysis of human primary fibroblasts and keratinocytes treated with or without trehalose.
|
| |
|
| Contributor(s) |
Muto J, Fukuda S, Watanabe K, Dai X, Tsuda T, Kiyoi T, Kameda K, Kawakami R, Mori H, Shiraishi K, Murakami M, Imamura T, Higashiyama S, Fujisawa Y, Mizukami Y, Sayama K |
| Citation(s) |
36609486 |
| |
| Submission date |
Sep 28, 2021 |
| Last update date |
Jan 12, 2023 |
| Contact name |
Jun Muto |
| E-mail(s) |
junmuto@m.ehime-u.ac.jp
|
| Organization name |
Ehime University Graduate School of Medicine
|
| Department |
Department of Dermatology
|
| Street address |
Shitsukawa 454
|
| City |
Toon |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (24)
|
| GSM5599689 |
2D fibroblasts_1 treated with trehalose for 24 hr |
| GSM5599690 |
2D fibroblasts_2 treated with trehalose for 24 hr |
| GSM5599691 |
2D fibroblasts_3 treated with trehalose for 24 hr |
| GSM5599692 |
2D fibroblasts_1 treated without trehalose |
| GSM5599693 |
2D fibroblasts_2 treated without trehalose |
| GSM5599694 |
2D fibroblasts_3 treated without trehalose |
| GSM5599695 |
3D fibroblasts_1 of final LSE with trehalose |
| GSM5599696 |
3D fibroblasts_2 of final LSE with trehalose |
| GSM5599697 |
3D fibroblasts_3 of final LSE with trehalose |
| GSM5599698 |
3D fibroblasts_1 of final LSE without trehalose |
| GSM5599699 |
3D fibroblasts_2 of final LSE without trehalose |
| GSM5599700 |
3D fibroblasts_3 of final LSE without trehalose |
| GSM5599701 |
3D keratinocyte_1 of final LSE with trehalose |
| GSM5599702 |
3D keratinocyte_2 of final LSE with trehalose |
| GSM5599703 |
3D keratinocyte_3 of final LSE with trehalose |
| GSM5599704 |
3D keratinocyte_1 of final LSE without trehalose |
| GSM5599705 |
3D keratinocyte_2 of final LSE without trehalose |
| GSM5599706 |
3D keratinocyte_3 of final LSE without trehalose |
| GSM5599707 |
3D fibroblasts_1 treated with trehalose for 72 hr |
| GSM5599708 |
3D fibroblasts_2 treated with trehalose for 72 hr |
| GSM5599709 |
3D fibroblasts_3 treated with trehalose for 72 hr |
| GSM5599710 |
3D fibroblasts_1 treated without trehalose |
| GSM5599711 |
3D fibroblasts_2 treated without trehalose |
| GSM5599712 |
3D fibroblasts_3 treated without trehalose |
|
| Relations |
| BioProject |
PRJNA766853 |
| SRA |
SRP339079 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE184892_RAW.tar |
62.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...