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| Status |
Public on Sep 23, 2021 |
| Title |
Gene expression data from patients with pancreatic ductal adenocarcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Pancreatic ductal adenocarcinoma (PDAC) is one of the most treatment-resistant malignancies in human. To perform transcriptomic profiling on PDAC, we collected primary PDAC tissues from surgically resected PDAC patients at the Memorial-Sloan Kettering Cancer Center (NY, USA). Morphogenesis of epithelial tissues relies on the precise developmental control of cell polarity and architecture. In the early Drosophila embryo, the primary epithelium forms during cellularisation, following a tightly controlled genetic programme where specific sets of genes are up-regulated. Some of them, for instance, control membrane invagination between the nuclei anchored at the apical surface of the syncytium.
We used microarrays to detail transcritomic profiling of PDACs.
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| Overall design |
Total RNA was extracted from fresh frozen OCT-embedded human PDAC tumor tissues using TRIxol RNA Isolation Reagent (Life Technologies). RNA was processed with the WT Pico Reagent Kit (Affymetrix) and T7 in vitro transcription thechnology into the cRNA, then hybridiezd to GeneChip Human Transcriptome Array 2.0 (Affymetrix), and scanned using the GeneChip Scanner 3000.
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| Contributor(s) |
Balachandran V, Moral AJ, Zhao J |
| Citation missing |
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| |
| Submission date |
Sep 22, 2021 |
| Last update date |
Sep 26, 2021 |
| Contact name |
Masataka Amisaki |
| E-mail(s) |
amisakim@mskcc.org
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| Phone |
6468882128
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| Organization name |
Memorial Sloan Kettering Cancer Center
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| Department |
Human oncology & pathogenesis program(HOPP)
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| Lab |
Balachandran lab
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| Street address |
1275 York Ave
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (82)
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| Relations |
| BioProject |
PRJNA765314 |
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