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| Status |
Public on Sep 29, 2021 |
| Title |
Microenvironmental IL-6 Inhibits Anti-Cancer Immune Responses Generated by Cytotoxic Chemotherapy |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA sequencing of tumor and stroma isolated from the bone marrow of wild-type and IL6 KO C57BL/6J mice
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| Overall design |
Bone marrow was isolated from dissected femur and tibia of WT and IL6KO mice. Bone marrow was depleted of red blood cells, then tumor and stromal cells were isolated by FACS. RNA was extracted and sequenced to a depth of at least 10 million reads per sample, with a read length of 75 nt. Reads were aligned and quantified by salmon, differentially expressed genes were identified by DESeq.
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| Contributor(s) |
Bent EH, Millán-Barea LR, Zhuang I, Goulet DR, Fröse J, Hemann MT |
| Citation(s) |
34711820 |
| |
| Submission date |
Sep 14, 2021 |
| Last update date |
Nov 04, 2021 |
| Contact name |
Daniel Robert Goulet |
| E-mail(s) |
dgoulet@mit.edu
|
| Phone |
8604831381
|
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Koch Institute
|
| Lab |
Michael T. Hemann
|
| Street address |
500 Main St
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA763190 |
| SRA |
SRP337037 |
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