|
| Status |
Public on Jan 28, 2022 |
| Title |
Cellular Atlas of Human Heart Failure |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Heart failure represents a major cause of morbidity and mortality worldwide. Single cell transcriptomics have revolutionized our understanding of cell composition and associated gene expression across human tissues. Through integrated analysis of single cell and single nucleus RNA sequencing data generated from 45 individuals, we define the cell composition of the healthy and failing human heart. We identify cell specific transcriptional signatures of heart failure and reveal the emergence of disease associated cell states. Intriguingly, cardiomyocytes converge towards a common disease associated cell state, while fibroblasts and myeloid cells undergo dramatic diversification. Endothelial cells and pericytes display global transcriptional shifts without changes in cell complexity. Collectively, our findings provide a comprehensive analysis of the cellular and transcriptomic landscape of human heart failure, identify cell type specific transcriptional programs and states associated with disease, and establish a valuable resource for the investigation of human heart failure.
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| Overall design |
Single cell n=7 (2 donor, 5 DCM), single nuclei n=38 (25 donor, 13 DCM).
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| Contributor(s) |
Koenig AL, Shchukina I, Andhey PS, Zaitsev K, Lai L, Amrute J, Bajpai G, Bredemeyer A, Smith G, Jones C, Terrebonne E, Rentschler SL, Artyomov MN, Lavine KJ |
| Citation(s) |
35959412 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL139714 |
MACROPHAGE ONTOGENY SHIFTS IN ISCHEMIC HEART DISEASE |
WASHINGTON UNIVERSITY |
Kory J. Lavine |
| R01 HL138466 |
MACROPHAGE HETEROGENEITY IN HEART FAILURE PROGRESSION AND CARDIAC RECOVERY |
WASHINGTON UNIVERSITY |
Kory J. Lavine |
| R01 HL151078 |
The Role of Donor Innate Immune Responses in Regulating Alloimmunity after Heart Transplantation |
WASHINGTON UNIVERSITY |
Kory J. Lavine |
| T32 HL134635 |
Training in Integrative and Systems Biology of Cardiovascular Disease |
WASHINGTON UNIVERSITY |
JEANNE M. NERBONNE |
| T32 HL125241 |
Pediatric Cardiovascular and Pulmonary Research Training Program |
WASHINGTON UNIVERSITY |
Juliane Bubeck Wardenburg |
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| |
| Submission date |
Sep 09, 2021 |
| Last update date |
Jul 24, 2023 |
| Contact name |
Kory Lavine |
| E-mail(s) |
klavine@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Internal Medicine - Cardiology
|
| Street address |
660 S. Euclid Ave
|
| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA762100 |
| SRA |
SRP336428 |
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