|
| Status |
Public on Sep 22, 2021 |
| Title |
Altered pattern of circulating miRNAs in HIV lipodystrophy perturbs key adipose differentiation and inflammation pathways |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified a microRNA (miRNA) profile characterizing HIV lipodystrophy and explored the downstream mechanistic implications with respect to adipocyte biology and the associated clinical phenotype. Human preadipocytes transfected with mimic miR-20a-3p, anti–miR-324-5p, or anti–miR-186 induced consistent changes in latent transforming growth factor beta binding protein 2 (Ltbp2), Wisp2, and Nebl expression.
|
| |
|
| Overall design |
Human preadipocytes were transfected with miR-20a-3p mimic, anti–miR-186, anti–miR-324-5p,or control nontargeting miRNA and then cultured in differentiation medium for 12 additional days to give rise to mature adipocytes, after which RNA was collected and subjected to RNA-Seq.
|
| |
|
| Contributor(s) |
Srinivasa S, Garcia-Martin R, Kahn CR, Grinspoon SK |
| Citation(s) |
34383714 |
| |
| Submission date |
Sep 09, 2021 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Hui Pan |
| E-mail(s) |
Hui.Pan@joslin.harvard.edu
|
| Organization name |
Joslin Diabetes Center
|
| Department |
Bioinformatics and Biostatistics Core
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA762058 |
| SRA |
SRP336401 |
Less...
More...