|
| Status |
Public on Sep 02, 2024 |
| Title |
Cardiac differentiation of mESCs and analysis of chromatin accessibility time series |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Assay for transposase-accessible chromatin-sequencing (ATAC-seq) was performed in a time-series of cardiac differentiation of mESCs
|
| |
|
| Overall design |
Two biological replicates were collected every 24 hours of cardiac differentiation of A2lox.cre mESCs from the 2i stage through day 8, in addition to day 10 and day 15 (cardiomyocyte stage). Samples were then lysed, and transposition was carried out at 37C for 30 minutes with Illumina’s Nextera DNA Library Prep kit (Illumina 15028212). Libraries were generated from transposed DNA and sequenced on an Illumina HiSeq4000 instrument in the University of Chicago’s Genomic Facility.
|
| |
|
| Contributor(s) |
Rowton MJ, Perez-Cervantes C, Lu E, Moskowitz IP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 09, 2021 |
| Last update date |
Sep 02, 2024 |
| Contact name |
Megan J Rowton |
| Organization name |
University of Chicago
|
| Department |
Human Genetics and Genetic Medicine
|
| Lab |
Moskowitz
|
| Street address |
900 East 57th Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA761997 |
| SRA |
SRP336353 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE183767_2i_FE.bw |
143.8 Mb |
(ftp)(http) |
BW |
| GSE183767_2i_peaks.narrowPeak.gz |
1.1 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D0_FE.bw |
155.5 Mb |
(ftp)(http) |
BW |
| GSE183767_D0_peaks.narrowPeak.gz |
926.2 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D10_FE.bw |
295.1 Mb |
(ftp)(http) |
BW |
| GSE183767_D10_peaks.narrowPeak.gz |
779.8 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D15_FE.bw |
272.0 Mb |
(ftp)(http) |
BW |
| GSE183767_D15_peaks.narrowPeak.gz |
884.1 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D1_FE.bw |
177.9 Mb |
(ftp)(http) |
BW |
| GSE183767_D1_peaks.narrowPeak.gz |
729.8 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D2_FE.bw |
225.8 Mb |
(ftp)(http) |
BW |
| GSE183767_D2_peaks.narrowPeak.gz |
951.6 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D3_FE.bw |
255.2 Mb |
(ftp)(http) |
BW |
| GSE183767_D3_peaks.narrowPeak.gz |
998.3 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D4_FE.bw |
196.7 Mb |
(ftp)(http) |
BW |
| GSE183767_D4_peaks.narrowPeak.gz |
1.2 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D5_FE.bw |
259.2 Mb |
(ftp)(http) |
BW |
| GSE183767_D5_peaks.narrowPeak.gz |
1.0 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D6_FE.bw |
365.8 Mb |
(ftp)(http) |
BW |
| GSE183767_D6_peaks.narrowPeak.gz |
834.3 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D7_FE.bw |
326.2 Mb |
(ftp)(http) |
BW |
| GSE183767_D7_peaks.narrowPeak.gz |
1.6 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE183767_D8_FE.bw |
357.7 Mb |
(ftp)(http) |
BW |
| GSE183767_D8_peaks.narrowPeak.gz |
1.3 Mb |
(ftp)(http) |
NARROWPEAK |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
Less...
More...