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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 10, 2021 |
| Title |
Real-time quantitative PCR analysis of mouse lungs after exposure to SARS-CoV-2 and variants of concern (VOC) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Age-matched K18-hACE2 transgenic mice were infected intranasally with different SARS-CoV-2 viruses, including (1) USA-WA1/2020 (WA) of lineage A, (2) New York-PV09158/2020 (NY) of lineage B.1.3, (3) USA/CA_CDC_5574/2020 (CA) of lineage B.1.1.7 and (4) hCoV-19/South Africa/KRISP-EC-K005321/2020 (SA) of lineage B.1.351. Mouse lungs were harvested on 3 days post infection (dpi). Total RNA was extracted using QIAgen RNeasy Plus Mini Kit and was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). Resultant cDNA was used as the template along with RT2 SYBR Green ROX qPCR Mastermix (Qiagen) to perform RT² Profiler™ PCR Array Mouse Hypoxia Signaling Pathway (Qiagen) real-time PCR in Stratagene MX3000p qPCR system.
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| Overall design |
qPCR gene expression profiling. Lung homogenates from 4-5 SARS-CoV-2 infected K18-hACE2 transgenic mice on dpi 3 were treated as indicated in the summary. Total RNA extracted was analyzed individually.
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| Contributor(s) |
Xie H, Radvak P |
| Citation(s) |
34772941 |
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| Submission date |
Sep 07, 2021 |
| Last update date |
Nov 13, 2021 |
| Contact name |
Hang Xie |
| E-mail(s) |
Hang.Xie@fda.hhs.gov
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| Organization name |
US Food and Drug Administration, Center for Biologics Evaluation and Research
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| Street address |
10993 New Hampshire Ave WO 52/72 Rm 1324
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| City |
Silver Spring |
| State/province |
Maryland |
| ZIP/Postal code |
20993 |
| Country |
USA |
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| Platforms (1) |
| GPL30599 |
QIAgen RT² Profiler PCR Array Mouse Hypoxia Signaling Pathway |
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| Samples (21)
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| GSM5563626 |
Lung, Naive (SAMPLE 4) |
| GSM5563627 |
Lung, WA (SAMPLE 5) |
| GSM5563628 |
Lung, WA (SAMPLE 6) |
| GSM5563629 |
Lung, WA (SAMPLE 7) |
| GSM5563630 |
Lung, WA (SAMPLE 8) |
| GSM5563631 |
Lung, WA (SAMPLE 9) |
| GSM5563632 |
Lung, NY (SAMPLE 10) |
| GSM5563633 |
Lung, NY (SAMPLE 11) |
| GSM5563634 |
Lung, NY (SAMPLE 12) |
| GSM5563635 |
Lung, NY (SAMPLE 13) |
| GSM5563636 |
Lung, CA (SAMPLE 14) |
| GSM5563637 |
Lung, CA (SAMPLE 15) |
| GSM5563638 |
Lung, CA (SAMPLE 16) |
| GSM5563639 |
Lung, CA (SAMPLE 17) |
| GSM5563640 |
Lung, SA (SAMPLE 18) |
| GSM5563641 |
Lung, SA (SAMPLE 19) |
| GSM5563642 |
Lung, SA (SAMPLE 20) |
| GSM5563643 |
Lung, SA (SAMPLE 21) |
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| Relations |
| BioProject |
PRJNA761417 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE183621_Fold_change.txt.gz |
3.6 Kb |
(ftp)(http) |
TXT |
| GSE183621_non-normalized_data.txt.gz |
4.1 Kb |
(ftp)(http) |
TXT |
| GSE183621_normalized_data.txt.gz |
9.7 Kb |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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