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| Status |
Public on May 10, 2024 |
| Title |
Next-Generation Sequencing to compare RNA transcripts between Adam19 wildtype and knockout mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
ADAM19 has been associated with pulmonary function traits in a number of genome wide association analyses by our group and others. We want to use a mouse model to examine the role Adam19 in lung function to clarify whether ADAM19 is truly the causal gene at this locus. We created a whole body Adam19 knockout mouse model targeting exon6 and 7 to disrupt the transcript expression from exon 8 through the end of gene sequences. In previous publications, mice lacking Adam19 exon 11, which harbor a catalytic site, were not postnatal viable. Contradictory to the previous publications, our Adam19 knockout survived postnatally. Our objectives were to determine if this novel mouse model is truly Adam19-deficient rather than a hypomorph or neomorph rescuing the previously published lethal phenotype of Adam19-deficient mice.
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| Overall design |
mRNA profiles of the heart and lung of wildtype and Adam19 knockout generated by deep sequencing, in triplicate, using Illumina NextSeq-High.
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| |
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| Contributor(s) |
Li H, Ward JM, London S, Gruzdev A |
| Citation(s) |
38659817, 39153120 |
| |
| Submission date |
Sep 02, 2021 |
| Last update date |
Oct 10, 2024 |
| Contact name |
Huiling Li |
| E-mail(s) |
li12@niehs.nih.gov
|
| Phone |
9842874249
|
| Organization name |
NIEHS
|
| Street address |
111 T.W. Alexander Dr
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA759920 |
| SRA |
SRP335436 |
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