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| Status |
Public on Jul 21, 2022 |
| Title |
Next generation sequencing facilitates quantitative analysis of transcriptomes of adult mouse alveolar epithelial type 2 (AT2) cells with Atf3 or Thra overexpression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study are to generate transcriptome profiling (RNA-seq) of AT2 cells with over expression of Atf3 and Thra.
Methods: AT2 cell mRNA profiles of 5 or 6-week-old wild-type mice were generated by deep sequencing, in triplicate. The Illumina Nextseq 500 system was used to generate raw data at 75 cycles. Fastq files was aligned to the reference genome and DEseq2 was used to analyze the differentially expressed genes.
Results: Our results show that Atf3 or Thra overexpression promotes the expression of genes associated AT2 cell proliferation.
Conclusions: Our study represents the first detailed analysis of transcriptomes of mouse AT2 cell with Atf3 or Thra overexpression, with biologic replicates, generated by RNA-seq technology.
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| Overall design |
Mouse AT2 cell mRNA profiles of Ad-Cre (Ctrl), Ad-Atf3, and Ad-Thra AT2 cells
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| Contributor(s) |
Ying T |
| Citation(s) |
35996586 |
| |
| Submission date |
Aug 31, 2021 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Ying Tian |
| E-mail(s) |
ying.tian@temple.edu
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| Phone |
215-707-7192
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| Organization name |
Lewis Katz School of Medicine at Temple University
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| Department |
Pharmacology, Center for Translational Medicine
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| Street address |
3500 N. Broad Street
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19146 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA759237 |
| SRA |
SRP335029 |
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