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| Status |
Public on Dec 01, 2021 |
| Title |
RNA-Seq analysis of Rpl22l1-deficient murine CD4-CD-CD44-CD25+ (DN3) thymocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Rpl22l1 is a highly conserved ribosomal protein paralog that plays a regulatory role in certain hematopoietic cell lineages. Rpl22l1-deficiency arrests the development of T cells at the DN3 stage. RNA-seq analysis was performed to identify the molecular basis for the arrest
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| Overall design |
Adult DN3 thymocytes were isolated from Vav-Cre- Rpl22l1fl/fl (L1 KO) and Vav-Cre+ Rpl22l1fl/fl mice (WT). Triplicate samples were lysed in Trizol. Total RNA was isolated and processed as previously described (Miyazaki et al., 2017).
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| Contributor(s) |
Wiest DL, Fahl SP |
| Citation(s) |
35046107 |
| |
| Submission date |
Aug 30, 2021 |
| Last update date |
Mar 02, 2022 |
| Contact name |
David Lynn Wiest |
| E-mail(s) |
david.wiest@fccc.edu
|
| Phone |
2157282966
|
| Organization name |
FOX CHASE CANCER CENTER
|
| Department |
Blood Cell Development and Function Program
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| Lab |
R364 Reimann Bldg.
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| Street address |
333 COTTMAN AVENUE, REIMANN BUILDING, LAB R364
|
| City |
PHILADELPHIA |
| State/province |
PA |
| ZIP/Postal code |
19111 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA758966 |
| SRA |
SRP334745 |
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