|
| Status |
Public on Aug 31, 2021 |
| Title |
Horizontal mitochondrial transfer from microenvironment increases glioblastoma tumorigenicity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Glioblastoma (GBM) cells acquire mitochondria from glial cells in vitro and in vivo. This transfer is accompanied by increased tumor-initiation capacity.
|
| |
|
| Overall design |
GFP+ SB28 tumor cells were co-cultured with CellTrace Violet-stained astrocytes from mito::mKate2 mice at a 1:1 ratio for 2 days. Tumors with or without exogenous mitochondria and donor astrocyte population were sorted based on CellTraceViolet-GFP-+mKate- (astrocyte), CellTraceViolet+GFP-mKate2-(mKate_neg) and CellTraceViolet+GFP-mKate2+(mKate_pos) from 3 biological replicates.
|
| |
|
| Contributor(s) |
Watson DC, Bayik D, Lathia JD |
| Citation(s) |
37169842 |
| |
| Submission date |
Aug 29, 2021 |
| Last update date |
Aug 11, 2023 |
| Contact name |
Yadi Zhou |
| Organization name |
Cleveland Clinic
|
| Street address |
9500 Euclid Ave
|
| City |
Cleveland |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA758703 |
| SRA |
SRP334624 |
Less...
More...