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| Status |
Public on Oct 13, 2021 |
| Title |
An AraC/XylS Family Transcriptional Regulator Modulates the Oxidative Stress Response of Francisella tularensis |
| Organism |
Francisella tularensis subsp. holarctica LVS |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Francisella tularensis is a Gram-negative bacterium that causes a fatal human disease known as tularemia. The Centers for Disease Control have classified F. tularensis as Category A Tier-1 Select Agent. The virulence mechanisms of Francisella are not entirely understood. Francisella possesses very few transcription regulators, and most of these regulate the expression of genes involved in intracellular survival and virulence. The F. tularensis genome sequence analysis reveals an AraC (FTL_0689) transcriptional regulator homologous to the AraC/XylS family of transcriptional regulators. In Gram-negative bacteria, AraC activates genes required for L-arabinose utilization and catabolism. The role of the FTL_0689 regulator in F. tularensis is not known. In this study, we characterized the role of FTL_0689 in gene regulation of F. tularensis and investigated its contribution to intracellular survival and virulence. The results demonstrate that FTL_0689 in Francisella is not required for L-arabinose utilization. Instead, FTL_0689 specifically regulates the expression of the oxidative and global stress response, virulence, metabolism, and other key pathways genes required by Francisella when exposed to oxidative stress. The FTL_0689 mutant is attenuated for intramacrophage growth, and mice infected with the FTL_0689 mutant survive better than wild-type F. tularensis LVS infected mice. Based on the deletion mutant phenotype, FTL_0689 was termed osrR (oxidative stress response regulator). Altogether, this study elucidates the role of the osrR transcriptional regulator in tularemia pathogenesis.
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| Overall design |
Comparison of transcript abundance in Francisella tularensis with or without the osrR gene when grown in the absence or the presence of oxidative stress induced by a superoxide generating compound, menadione.
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| Contributor(s) |
Marghani D, Ma Z, Huang W, Malik M, Bakshi CS |
| Citation(s) |
34543107 |
| |
| Submission date |
Aug 29, 2021 |
| Last update date |
Jan 17, 2022 |
| Contact name |
Weihua Huang |
| E-mail(s) |
huangwe21@ecu.edu
|
| Phone |
2527443748
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| Organization name |
East Carolina University
|
| Department |
Pathology
|
| Street address |
600 Moye Blvd, Brody 7S-24
|
| City |
Greenville |
| State/province |
North Carolina |
| ZIP/Postal code |
27834 |
| Country |
USA |
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| Platforms (1) |
| GPL23586 |
Illumina MiSeq (Francisella tularensis subsp. holarctica LVS) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA758701 |
| SRA |
SRP334622 |
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