 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 05, 2022 |
| Title |
Mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistance: PTBP1 iCLIP2 in NALM-6 cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We developed a massively parallel reporter assay to determine the splicing effects of all mutations in the region comprising CD19 exons 1-3. Despite the great success of CART-19 (chimeric antigen receptor-armed autologous T-cells) immunotherapy to treat B-cell acute lymphoblastic leukaemia (B-ALL), many patients relapse due to loss of the targeted CD19 epitope. Since epitope loss can be caused by CD19 exon 2 mis-splicing, we set out to learn the regulatory code that controls CD19 alternative splicing.
|
| |
|
| Overall design |
iCLIP2 protocol performed in NALM-6 cell lines in two replicates under two different RNAse I dilution values
|
| |
|
| Contributor(s) |
Cortés-López M, Schulz L, Enculescu M, Paret C, Spiekermann B, Busch A, Orekhova A, Kielisch F, Quesnel-Vallières M, Torres-Diz M, Faber J, Barash Y, Thomas-Tikhonenko A, Zarnack K, Legewie S, König J |
| Citation(s) |
36138008 |
| |
| Submission date |
Aug 26, 2021 |
| Last update date |
Sep 28, 2022 |
| Contact name |
Julian König |
| E-mail(s) |
j.koenig@imb-mainz.de
|
| Organization name |
Institute of Molecular Biology, Mainz
|
| Department |
Biology
|
| Lab |
König
|
| Street address |
Ackermannweg 4
|
| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE182894 |
Mutations and RNA-binding proteins controlling CD19 splicing and CART-19 therapy resistance |
|
| Relations |
| BioProject |
PRJNA758172 |
| SRA |
SRP334381 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE182893_RAW.tar |
131.8 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...