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| Status |
Public on Aug 27, 2021 |
| Title |
Antigen-receptor stimulation drives purifying selection against pathogenic mitochondrial DNA variants that dysregulate lymphocyte responses |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Metabolic transcriptomic signatures of CD8+ T cells during timecourse activation were profiled in CD8 T cells isolated from 4 WT and 4 C5024T mice. Mice were age (2-month-old) and sex matched. Briefly, for each mouse, freshly purified (negative selection) splenic naïve CD8+ T cells were seeded at a density of 200,000 cells/well and activated using a 1:1 (bead:cell) ratio with Dynabeads Mouse T-Activator CD3/CD28 for 6 and 96 hr at 37oC (5% CO2) . 200,000 ex vivo purify ed naïve CD8+ T cells were collected for baseline (0 hr) measurement.
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| Overall design |
4wt and 4 C5024T mice,from each mice, sub-divided into three groups: naive CD8 T cell, CD8 T cell post_6h activation, CD8 T cell_post 96h activation.
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| Contributor(s) |
Zhang J, Castro Dopico X, Rorbach J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 26, 2021 |
| Last update date |
Aug 29, 2021 |
| Contact name |
Joanna Rorbach |
| E-mail(s) |
joanna.rorbach@ki.se
|
| Phone |
+46763142079
|
| Organization name |
karolinska institute
|
| Department |
Department of Medical Biochemistry and Biophysics
|
| Lab |
Joanna Rorbach Lab
|
| Street address |
Solnavägen 9, 9d 171 77 Stockholm, Sweden
|
| City |
Stockholm |
| State/province |
Not Applicable (Outside of US and Canada) |
| ZIP/Postal code |
171 77 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL30557 |
NanoString nCounter Mouse Metabolic Pathways Panel |
|
| Samples (23)
|
| GSM5542537 |
C5024T(Ala) naive CD8 T cell_Biological repeats_ A1 |
| GSM5542538 |
C5024T(Ala) CD8 T cell_post act 96h_Biological repeats_ A1 |
| GSM5542539 |
C5024T(Ala) naive CD8 T cell_Biological repeats_ A2 |
| GSM5542540 |
C5024T(Ala) CD8 T cell_post act 96h_Biological repeats_ A2 |
| GSM5542541 |
C5024T(Ala) CD8 T cell_post act 6h_Biological repeats_ A2 |
| GSM5542542 |
WT naive CD8 T cell_Biological repeats_ W1 |
| GSM5542543 |
WT CD8 T cell_post act 96h_Biological repeats_ W1 |
| GSM5542544 |
WT CD8 T cell_post act 6h_Biological repeats_ W1 |
| GSM5542545 |
WT naive CD8 T cell_Biological repeats_ W2 |
| GSM5542546 |
WT CD8 T cell_post act 96h_Biological repeats_ W2 |
| GSM5542547 |
WT CD8 T cell_post act 6h_Biological repeats_ W2 |
| GSM5542548 |
C5024T(Ala) naive CD8 T cell_Biological repeats_ A3 |
| GSM5542549 |
C5024T(Ala) CD8 T cell_post act 96h_Biological repeats_ A3 |
| GSM5542550 |
C5024T(Ala) CD8 T cell_post act 6h_Biological repeats_ A3 |
| GSM5542551 |
C5024T(Ala) naive CD8 T cell_Biological repeats_ A4 |
| GSM5542552 |
C5024T(Ala) CD8 T cell_post act 96h_Biological repeats_ A4 |
| GSM5542553 |
C5024T(Ala) CD8 T cell_post act 6h_Biological repeats_ A4 |
| GSM5542554 |
WT naive CD8 T cell_Biological repeats_ W3 |
| GSM5542555 |
WT CD8 T cell_post act 96h_Biological repeats_ W3 |
| GSM5542556 |
WT CD8 T cell_post act 6h_Biological repeats_ W3 |
| GSM5542557 |
WT naive CD8 T cell_Biological repeats_ W4 |
| GSM5542558 |
WT CD8 T cell_post act 96h_Biological repeats_ W4 |
| GSM5542559 |
WT CD8 T cell_post act 6h_Biological repeats_ W4 |
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| Relations |
| BioProject |
PRJNA758153 |
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