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| Status |
Public on Sep 28, 2022 |
| Title |
Transcriptomics in olfactory cells transfected with SARS-CoV-2 structural proteins |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
One of the most common symptoms in COVID-19 is a sudden loss of smell. SARS-CoV-2 has been detected in the olfactory bulb (OB) from animal models and sporadically in COVID-19 patients. To decipher the specific role of SARS-CoV-2 proteome at olfactory level, we deeply characterized the molecular imbalance induced by the expression of GFP-tagged SARS-CoV-2 structural proteins (M, N, E, S) on mouse OB cells by RNA-seq.
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| |
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| Overall design |
mouse OBC1 cells total RNA profile of 5 group of samples: M, E, N, S and control GFP group. Three replicates per sample. Deep sequencing in Illumina HiSeq1500.
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| Contributor(s) |
Santamaría E, Lachén-Montes M, Mendizuri N, Kochan G, de Toro M, Fernandez-Irigoyen J, Escors D |
| Citation(s) |
36159830 |
| |
| Submission date |
Aug 26, 2021 |
| Last update date |
Sep 29, 2022 |
| Contact name |
María de Toro |
| E-mail(s) |
mthernando@riojasalud.es
|
| Organization name |
Centro de Investigación Biomédica de La Rioja
|
| Department |
Genomics & Bioinformatics Core Facility
|
| Street address |
C/Piqueras 98
|
| City |
Logrono |
| State/province |
La Rioja |
| ZIP/Postal code |
26006 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
|
| Samples (15)
|
| GSM5538277 |
Induced sample by the SARS-CoV-2 Envelope (E) protein replicate 1 |
| GSM5538278 |
Induced sample by the SARS-CoV-2 Envelope (E) protein replicate 2 |
| GSM5538279 |
Induced sample by the SARS-CoV-2 Envelope (E) protein replicate 3 |
| GSM5538280 |
Induced sample by the SARS-CoV-2 Membrane (M) protein replicate 1 |
| GSM5538281 |
Induced sample by the SARS-CoV-2 Membrane (M) protein replicate 2 |
| GSM5538282 |
Induced sample by the SARS-CoV-2 Membrane (M) protein replicate 3 |
| GSM5538283 |
Induced sample by the SARS-CoV-2 Spike (S) protein replicate 1 |
| GSM5538284 |
Induced sample by the SARS-CoV-2 Spike (S) protein replicate 2 |
| GSM5538285 |
Induced sample by the SARS-CoV-2 Spike (S) protein replicate 3 |
| GSM5538286 |
Induced sample by the SARS-CoV-2 Nucleocapside (N) protein replicate 1 |
| GSM5538287 |
Induced sample by the SARS-CoV-2 Nucleocapside (N) protein replicate 2 |
| GSM5538288 |
Induced sample by the SARS-CoV-2 Nucleocapside (N) protein replicate 3 |
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| Relations |
| BioProject |
PRJNA758061 |
| SRA |
SRP334255 |
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