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Series GSE182746 Query DataSets for GSE182746
Status Public on Jun 17, 2022
Title Construction of the axolotl cell landscape using combinatorial hybridization sequencing at single cell resolution [bulk RNA-seq]
Organism Ambystoma mexicanum
Experiment type Expression profiling by high throughput sequencing
Summary We adopted a combinatorial hybridization based single-cell RNA-seq method to generate tissue based transcriptome atlas of adult axolotl and whole organism transcriptome atlas of larva axolotl. Gene expression profiling of over 1million single cells across 19 organs constructed the first adult axolotl cell atlas. Comparison between neoteny and metamorphosis organs revealed transcriptome heterogeneity of structural cells in different tissues and a sophisticated regulatory network. Furthermore, we described dynamic gene expression pattern during neotenic larva axolotl limb development. These data serve as a rich resource to explore molecular identity of axolotl as well as its metamorphosis.
 
Overall design Bulk RNA sequencing datasets for seventeen adult neotenic axolotl tissues (brain, bladder, cloaca, eye, fore limb, hind limb, gill, gonad, heart, intestine, kidney, liver, lung, skin, spleen, stomach, tail) and fourteen adult metamorphosed axolotl tissues (bladder, brain, cloaca, eye, hind limb, heart, intestine, kidney, liver, lung, skin, spleen, stomach, tail). Single cell combinatorial hybridization RNA sequencing datasets for nineteen adult neotenic axolotl tissues (brain, bladder, cloaca, eye, fore limb, hind limb, gill, gonad, pancreas, prostate, heart, intestine, kidney, liver, lung, skin, spleen, stomach, tail), sixteen adult metamorphosed axolotl tissues ( bladder, brain, cloaca, eye, fore limb, hind limb, heart, intestine, kidney, liver, lung, skin, spleen, stomach, tail, gonad) and neotenic larva stage axolotl whole organism covering 5 stages including Day 30, Day 35, Day 45, Day 50, Day 70 postfertilization.
 
Contributor(s) Guo G, Han X, Ye F, Ee W, Li J, Fu Y
Citation(s) 35869072
Submission date Aug 25, 2021
Last update date Jul 26, 2022
Contact name Fang Ye
E-mail(s) 11618108@zju.edu.cn
Phone +86 18167144848
Organization name Zhejiang University
Street address 866 Yuhangtang Rd
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310058
Country China
 
Platforms (1)
GPL30540 HiSeq X Ten (Ambystoma mexicanum)
Samples (61)
GSM5536000 Bulk-RNA-seq dataset of neotenic axolotl bladder (Batch1)
GSM5536001 Bulk-RNA-seq dataset of neotenic axolotl bladder (Batch2)
GSM5536002 Bulk-RNA-seq dataset of neotenic axolotl brain (Batch1)
Relations
BioProject PRJNA757699
SRA SRP334094

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE182746_RAW.tar 111.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record
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