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| Status |
Public on Aug 01, 2024 |
| Title |
Influenza-induced activation of recruited alveolar macrophages during the early inflammatory phase drives lung injury and lethality |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
we identify gene expression patterns associated with three distinct phases (Infiltrating, Early Inflammatory, Late Inflammatory) in the influenza A virus response of recruited alveolar macrophages. In the Early Inflammatory phase, recruited macrophages begin expressing pro-inflammatory genes, driving cytokine-mediated lung damage and contributing to the morbidity observed in influenza-infected wild-type mice. In mice lacking vimentin (Vim-/-), genes associated with the Early Inflammatory phase are suppressed, while the expression of the Infiltrating Phase genes is maintained, which is associated with protection from influenza-induced injury. Using a bone-marrow chimera mouse model, we validate that Vim-/- recruited alveolar macrophages are sufficient to confer protection from influenza-induced mortality. Our results define the temporal dynamics of gene expression in recruited alveolar macrophages, which shape the host response to infection with respiratory viruses.
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| Overall design |
RNA-seq on MoAMs over a 6-day time-course following IAV infection to characterize the immune response
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| Contributor(s) |
M Koch C, Anekalla KR, R Winter D, M Ridge K |
| Citation missing |
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| |
| Submission date |
Aug 24, 2021 |
| Last update date |
Aug 01, 2024 |
| Contact name |
kishore Anekalla |
| E-mail(s) |
kishore.anekalla@northwestern.edu
|
| Phone |
7327899423
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| Organization name |
Northwestern University
|
| Department |
Pulmonary and Critical Care
|
| Street address |
303 E. Superior
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60610 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA757241 |
| SRA |
SRP333843 |
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