|
| Status |
Public on Jan 02, 2022 |
| Title |
Clostridium tetani biomarkers for culture condition |
| Organism |
Clostridium tetani |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used RNA-seq to determine Clostridium tetani gene expression changes in response to culture conditions and time. Changes in response to time were more pronounced than those in response to culture conditions. The tetanus toxin gene is always highly expressed but does show expression changes between culture conditions. These results may become part of an approach to reduce animal testing during vaccine manufacturing.
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| |
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| Overall design |
39 samples, comprising 6 growth conditions and 5 time points, with some replicates.
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| |
|
| Contributor(s) |
Pennings J |
| Citation(s) |
35051008 |
| |
| Submission date |
Aug 19, 2021 |
| Last update date |
Jan 21, 2022 |
| Contact name |
Jeroen Pennings |
| E-mail(s) |
Jeroen.Pennings@rivm.nl
|
| Phone |
+31 88 689 2214
|
| Organization name |
Natl. Inst. Public Health & Environment
|
| Street address |
A. van Leeuwenhoeklaan 9
|
| City |
Bilthoven |
| ZIP/Postal code |
3721MA |
| Country |
Netherlands |
| |
|
| Platforms (2) |
| GPL28078 |
Illumina MiSeq (Clostridium tetani) |
| GPL30527 |
Illumina NextSeq 500 (Clostridium tetani) |
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| Samples (39)
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| Relations |
| BioProject |
PRJNA756180 |
| SRA |
SRP333280 |
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