|
Status |
Public on Nov 09, 2009 |
Title |
New candidate gene identification for controlling mammalian gonadal sex determination |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
Mammalian gonadal sex determination is dependent on proper expression of sex determining genes in fetal gonadal somatic support cells (i.e., pre-granulosa and pre-Sertoli cells in XX and XY gonads, resp.). We used a unique transgenic mouse strain combined with microarray profiling to identify all the differentially expressed transcripts in XX and XY isolated somatic support cells during critical stages of gonadal development and differentiation.
|
|
|
Overall design |
XX and XY somatic support cells (SSC) were isolated by flow cytometry from embryonic day (E) 11.5 and E12.5 mouse gonads. Total RNA was isolated from pools of isolated cells; 3 pools per sex and each timepoint.
|
|
|
Contributor(s) |
Bouma GJ |
Citation(s) |
19864314 |
|
Submission date |
Sep 22, 2009 |
Last update date |
Feb 11, 2019 |
Contact name |
Gerrit J Bouma |
E-mail(s) |
Gerrit.Bouma@colostate.edu
|
Organization name |
Colorado State University
|
Department |
Biomedical Sciences
|
Street address |
3801 W. Rampart Road
|
City |
Fort Collins |
State/province |
CO |
ZIP/Postal code |
80523 |
Country |
USA |
|
|
Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
Samples (12)
|
|
Relations |
BioProject |
PRJNA119579 |