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| Status |
Public on Aug 16, 2021 |
| Title |
Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitor [CUT&RUN] |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Yolk sac (YS) hematopoiesis is critical for the survival of the embryo and a major source of tissue-resident macrophages that persist into adulthood. Here we report that the epigenetic regulator Ezh2 is essential for YS hematopoiesis but dispensable for subsequent aorta–gonad–mesonephros (AGM) blood development. Loss of Ezh2 activity in hemogenic endothelium (HE) leads to the generation of phenotypically intact but functionally deficient erythro-myeloid progenitors (EMP). Ezh2 activity is critical at the onset of the endothelial-to-hematopoietic transition generating EMPs but rapidly dispensable after that. We identified a lack of downregulation of Wnt signaling as the primary reason in the generation of non-functional EMPs. Together, our findings demonstrate a critical and specific role of Ezh2 in modulating Wnt signaling during the generation of EMPs from YS HE.
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| Overall design |
RNA-seq, ATAC-seq and CUT&RUN profile of E10.5 yolk sac erythro-myeloid progenitors
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| |
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| Contributor(s) |
Neo WH, Meng Y, Rodriguez-Meira A, Fadlullah MZ, Booth CA, Azzoni E, Thongjuea S, de-Bruijn MF, Jacobsen SE, Mead AJ, Lacaud G |
| Citation(s) |
34857757 |
| |
| Submission date |
Aug 11, 2021 |
| Last update date |
Dec 06, 2021 |
| Contact name |
Georges Lacaud |
| E-mail(s) |
georges.lacaud@cruk.manchester.ac.uk
|
| Organization name |
Cancer Research UK Manchester Institute
|
| Department |
Stem Cell Biology
|
| Street address |
Alderley Park
|
| City |
Alderley Edge |
| State/province |
England |
| ZIP/Postal code |
SK10 4TG |
| Country |
United Kingdom |
| |
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| Platforms (2) |
| GPL21626 |
NextSeq 550 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE181873 |
Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors. |
|
| Relations |
| BioProject |
PRJNA753786 |
| SRA |
SRP332064 |
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