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Series GSE18168 Query DataSets for GSE18168
Status Public on Dec 01, 2009
Title Uterine gene profiles from WT, KIKO (DNA-binding deficient ERα) and aERKO mice treated with estradiol or xenoestrogens
Organism Mus musculus
Experiment type Expression profiling by array
Summary Ovariectomized WT, KIKO (DNA-binding deficient ERα) or αERKO female mice were injected (ip) with saline (vehicle), estradiol (E2; 250 ng), bisphenol A (BPA; 750 µg) or 2,2-bis(p-hydroxyphenyl)-1,1,1-trichloroethane (HPTE; 750 µg) and uteri were collected after 2 or 24 hours. Uterine profiles were compared and indicated the early (2 hour) responses to E2 were highly correlated to the BPA and HPTE profiles. KIKO patterns included overlap with WT but also included distinct responses. Later (24 hour) responses in the KIKO were weaker, indicating DNA binding is needed to maintain the estrogenic response. BPA and HPTE also showed a weakened late response in the WT, suggesting they are impeded estrogens.
Overall design Three WT, KIKO or αERKO ovariectomized mice per treatment group were injected with vehicle, E2, BPA or HPTE and uterine tissue was collected after 2 or 24 hour and frozen in liquid nitrogen. RNA was isolated from a pool of the three uteri using Trizol reagent and then cleaned with a Qiagen RNA easy mini kit. RNA quality was validated with the Agilent 2100 Bioanalyzer in the microarray facility. RNA samples were then analyzed by 2-color Agilent Whole Mouse arrays with fluor flips. Each array was co hybridized with vehicle and treated RNA within the genotype (WT, KIKO or aERKO).
Contributor(s) Hewitt S, Korach KS, Jameson JL, O'Brien J
Citation(s) 19812388, 20826375
Submission date Sep 18, 2009
Last update date May 10, 2018
Contact name NIEHS Microarray Core
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
Platforms (2)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (36)
GSM453672 E2_2h vs. WT V
GSM453673 aERKO V vs._E2_2h
GSM453674 E2_2h vs. aERKO V
BioProject PRJNA119519

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18168_RAW.tar 1.5 Gb (http)(custom) TAR (of TIFF, TXT)
GSE18168_US22502532_251486813434_S01_H.tif.gz 123.1 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486813434_S01_L.tif.gz 91.5 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486813435_S02_H.tif.gz 124.0 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486813435_S02_L.tif.gz 92.6 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486813436_S02_H.tif.gz 122.0 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486813436_S02_L.tif.gz 90.6 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486813438_S01_H.tif.gz 119.8 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486813438_S01_L.tif.gz 88.4 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486813439_S01_H.tif.gz 121.4 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486813439_S01_L.tif.gz 90.0 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486814897_S01_H.tif.gz 121.0 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486814897_S01_L.tif.gz 89.1 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486814898_S01_H.tif.gz 121.0 Mb (ftp)(http) TIFF
GSE18168_US22502532_251486814898_S01_L.tif.gz 89.4 Mb (ftp)(http) TIFF
Processed data included within Sample table

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