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| Status |
Public on Mar 12, 2024 |
| Title |
Gene expression profiles of mouse derived esophagus and stomach epithelial stem cells 3D organoids |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The gastroesophageal squamocolumnar junction (GE-SCJ) is a critical tissue interface between the esophagus and stomach, with significant relevance in the pathophysiology of gastrointestinal diseases. Despite this, the molecular mechanisms underlying GE-SCJ development remain unclear. Using single-cell transcriptomics, organoids, and spatial analysis, we examine the cellular heterogeneity and spatiotemporal dynamics of GE-SCJ development from embryonic to adult mice. We identify distinct transcriptional states and signaling pathways in the epithelial and mesenchymal compartments of the esophagus and stomach during development. Fibroblast-epithelial interactions are mediated by various signaling pathways, including WNT, BMP, TGF-β, FGF, EGF, and PDGF. Our results suggest that fibroblasts predominantly send FGF and TGF-β signals to the epithelia, while epithelial cells mainly send PDGF and EGF signals to fibroblasts. We observe differences in the ligands and receptors involved in cell-cell communication between the esophagus and stomach. Our findings provide insights into the molecular mechanisms underlying GE-SCJ development and fibroblast-epithelial crosstalk involved, paving the way to elucidate mechanisms during adaptive metaplasia development and carcinogenesis.
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| Overall design |
Mouse-derived esophagus and stomach epithelial stem cells were isolated and cultured as 3D organoids as described in the accompanying manuscript (Materials and Methods). Total RNA was extracted from samples and hybridized on Agilent-8x60k Mouse custom arrays as single-color hybridizations.
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| Contributor(s) |
Kumar Nirchal N, Gurumurthy R, Wentland C, Mollenkopf H, Chumduri C |
| Citation(s) |
38594232 |
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| Submission date |
Aug 03, 2021 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Cindrilla Chumduri |
| E-mail(s) |
cindrilla.chumduri@uni-wuerzburg.de
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| Organization name |
Julius-Maximilians-Universität Würzburg
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| Department |
Chair of Microbiology, Biozentrum
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| Street address |
Am Hubland
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| City |
Würzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA751904 |
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