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| Status |
Public on Feb 21, 2023 |
| Title |
Identification of the key epigenetic determinants of chondrocyte aging in humans (Single cell-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Articular chondrocytes undergo functional changes and their regenerative potential declines with age. Although the molecular mechanisms guiding articular cartilage aging is poorly understood, DNA methylation is known to play a mechanistic role in aging. However, our understanding of DNA methylation in chondrocyte development across human ontogeny is limited. To better understand DNA methylome changes, methylation profiling was performed in human chondrocytes. This study reveals association between methylation of specific CpG sites and chondrocyte age. We also determined the putative binding targets of STAT3, a key age-patterned TF in fetal chondrocytes and genetic ablation of STAT3 induced a global genomic hypermethylation. Moreover, an epigenetic clock built for adult human chondrocytes revealed that exposure of aged adult human chondrocytes to STAT3 agonist, decreased epigenetic age. Taken together, this work will serve as a foundation to understand development and aging of chondrocytes with a new perspective for development of rejuvenation agents for synovial joints.
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| |
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| Overall design |
Single-cell mRNA sequencing for human osteoarthritic chondrocytes using 10x Genomics
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| Contributor(s) |
Evseenko D, Van Handel B, Sarkar A |
| Citation(s) |
36638270 |
| |
| Submission date |
Aug 02, 2021 |
| Last update date |
Feb 22, 2023 |
| Contact name |
Denis Evseenko |
| E-mail(s) |
evseenko@usc.edu
|
| Phone |
323-219-0234
|
| Organization name |
University of Southern California
|
| Department |
Orthopaedic Surgery
|
| Lab |
Evseenko Lab
|
| Street address |
1450 Biggy St
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE181356 |
Identification of the key epigenetic determinants of chondrocyte aging in humans |
|
| Relations |
| BioProject |
PRJNA751624 |
| SRA |
SRP330945 |
