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| Status |
Public on Apr 14, 2022 |
| Title |
lncSMaRT translational regulation of Spire1, a new player in muscle differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Total RNA of DHX36 RIP experiment in Human differentiating myoblasts at DM1 was extracted and subjected to unstranded paired end library prep and sequencing. DHX36 is an ATP-dependent helicase of the DEAH family that is known to be involved in unwinding of G-Quadruplex structures, regulating mRNA metabolism and translation. We demonstrated that mouse DHX36 and the lncRNA lnc-SMaRT are involved in muscle differentiation by translationally regulating G-Quadruplex containing mRNAs (Mlx and Spire1). Here, we characterize the human DHX36 interactome in differentiating myoblasts in order to find out a lnc-SMaRT analogous gene.
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| Overall design |
INPUT, DHX36 IP and IgG samples were collected from human myoblasts at day 1 of differentiation
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| Contributor(s) |
Silvia S, Adriano S, Alessio C, Michela L, Davide M, Julie M |
| Citation(s) |
34863993 |
| |
| Submission date |
Aug 02, 2021 |
| Last update date |
Apr 15, 2022 |
| Contact name |
Irene Bozzoni |
| E-mail(s) |
irene.bozzoni@uniroma1.it
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| Organization name |
Sapienza University of Rome
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| Department |
Biology and Biotechnologies "Charles Darwin"
|
| Lab |
Bozzoni
|
| Street address |
Piazzale Aldo Moro 5
|
| City |
Rome |
| ZIP/Postal code |
00185 |
| Country |
Italy |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA751547 |
| SRA |
SRP330850 |
