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| Status |
Public on Aug 03, 2021 |
| Title |
Single-cell RNA sequencing of peripheral blood reveals immune cell signatures in Alzheimer's disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
This study aimed to explore the molecular basis of peripheral immune cells and the features of adaptive immune repertoire at a single cell level. We proļ¬led 36,849 peripheral blood mononuclear cells from AD patients with amyloid-positive status and normal controls with amyloid-negative status by 5' single-cell transcriptome and immune repertoire sequencing using the cell ranger standard analysis procedure. We revealed five immune cell subsets, CD4+ T cells, CD8+ T cells, B cells, natural killer cells, and monocytes-macrophages cells, and disentangled the characteristic alterations of cell subset proportion and gene expression patterns in AD. Thirty-one cell type-specific key genes, comprising abundant human leukocyte antigen genes, and multiple immune-related pathways were identified by protein-protein interaction network and pathway enrichment analysis. We also found high-frequency amplification clonotypes in T cells and B cells and decreased diversity in T cells in AD. As clone amplification suggested the activation of an adaptive immune response against specific antigens, we speculated that the peripheral adaptive immune response, especially mediated by T cells, may have a role in the pathogenesis of AD. This finding may also contribute to further research regarding disease mechanism and the development of immune-related biomarkers or therapy.
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| Overall design |
Three patients with AD and two cognitively normal controls (NC)
>>>Submitter states: The analysis of TCR-seq and BCR-seq has not yet been performed<<<
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| Contributor(s) |
Xu H, Jia JP |
| Citation(s) |
34447367 |
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| Submission date |
Aug 01, 2021 |
| Last update date |
Aug 31, 2021 |
| Contact name |
Hui Xu |
| E-mail(s) |
xuhui1517@126.com
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| Phone |
15176344607
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| Organization name |
Xuanwu Hospital
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| Department |
Department of neurology
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| Street address |
Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases
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| City |
Beijing |
| ZIP/Postal code |
100053 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA751391 |
| SRA |
SRP330776 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE181279_RAW.tar |
191.3 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
| GSE181279_barcodes.tsv.gz |
179.1 Kb |
(ftp)(http) |
TSV |
| GSE181279_features.tsv.gz |
302.7 Kb |
(ftp)(http) |
TSV |
| GSE181279_matrix.mtx.gz |
186.1 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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