|
| Status |
Public on Sep 17, 2023 |
| Title |
Non-coding small RNA expression analysis of rat pancreatic acinar cells(AR42J) treated with sodium taurocholate |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Acute pancreatitis (AP) is a common digestive disorder with high morbidity and mortality. At present, the pathogenic mechanisms of AP remain unclear. Pancreatic acinar intracellular trypsinogen activation is considered to be an important cause of AP and is an important event in the early stages of AP. The activation of trypsinogen is a key factor for the pancreas to maintain normal function and that the abnormal activation of trypsinogen in pancreatic acinar cells is an initiating factor for the occurrence of AP. In the past decade, microRNA-related research results suggest that small non-coding RNAs play an important role in AP. Recently, endogenous transfer RNA-derived small RNA (tsRNA), a newly identified non-coding small RNA, is reported to be associated with multiple diseases. tsRNAs can be broadly classified into two main groups: tiRNAs (tRNA halves) and tRFs (tRNA-derived fragments). tiRNAs are produced by specific cleavage in the anticodon loop under various stress conditions (29-50 nucleotides). tRFs are separated to 4 subtypes by their sites of origin in pre-tRNA or mature tRNA, and generally shorter than tiRNAs (16-28 nucleotides). Similar to the function of microRNA, tsRNA can inhibit their functions by binding to target genes. However, the role of tsRNA in regulating AP pathogenesis has not been investigated. In this experiment, sodium taurocholate was used to treat the rat pancreatic acinar cell (AR42J) to establish the AP-related intracellular activation of trypsinogen model. Then we used RNA sequencing to identify the differentially expressed non-coding small RNAs including microRNA and tsRNA in the cell model.
|
| |
|
| Overall design |
Non-coding small RNA profiles from AR42J cell line treated with 200 μM sodium taurocholate for 30min and their untreated controls were sequenced in an Illumina NextSeq.
|
| |
|
| Contributor(s) |
Gao B, Xue D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 29, 2021 |
| Last update date |
Sep 18, 2023 |
| Contact name |
Bo Gao |
| E-mail(s) |
bo.gaomichael@hotmail.com
|
| Organization name |
Peking University People's Hospital
|
| Department |
General Surgery
|
| Street address |
No.11 Xizhenmen South Street
|
| City |
Beijing |
| ZIP/Postal code |
100035 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA750677 |
| SRA |
SRP330427 |
Less...
More...