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Status |
Public on Aug 01, 2021 |
Title |
RNA seq analysis of MC38CEA cells transduced with pLKO1 vectors encoding shRNAs targeting Adam17 or control shRNA |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The goal of this study was to compare transcriptomes of cells with reduced and normal levels of ADAM17, unstimulated or stimulated with IL1b and IFNg
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Overall design |
The cells were transduced with pLKO vectors from MISSION® library encoding shRNA targeting mouse ADAM17: SL2 (TRCN0000031953) or control non-targeting shRNA: SHC016. Transduced cells were selected with puromycin. The cells were left unstimulated or were stimulated with human recombinant (hr) IL1b (10 ng/ml) and mouse recombinant (mr) IFNg (10 ng/ml) for six hours and RNA was isolated. Note: Control shRNA, SHC016, is cytotoxic and its expression leads to elimination of shRNA-coding casette (personal observation).
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Contributor(s) |
Czarnek M, Bereta J |
Citation(s) |
35107128 |
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Submission date |
Jul 29, 2021 |
Last update date |
Mar 15, 2022 |
Contact name |
Joanna Bereta |
E-mail(s) |
joanna.bereta@uj.edu.pl
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Phone |
+48-12-664-6356
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Organization name |
Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
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Department |
Cell Biochemistry
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Street address |
Gronostajowa 7
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City |
Kraków |
ZIP/Postal code |
31-387 |
Country |
Poland |
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Platforms (1) |
GPL18635 |
Ion Torrent Proton (Mus musculus) |
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Samples (12)
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Relations |
BioProject |
PRJNA750655 |
SRA |
SRP330415 |
Supplementary file |
Size |
Download |
File type/resource |
GSE181084_shRNA_RNAseq.xls.gz |
4.8 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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