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| Status |
Public on Aug 01, 2021 |
| Title |
RNA seq analysis of MC38CEA cells transduced with pLKO1 vectors encoding shRNAs targeting Adam17 or control shRNA |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study was to compare transcriptomes of cells with reduced and normal levels of ADAM17, unstimulated or stimulated with IL1b and IFNg
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| Overall design |
The cells were transduced with pLKO vectors from MISSION® library encoding shRNA targeting mouse ADAM17: SL2 (TRCN0000031953) or control non-targeting shRNA: SHC016. Transduced cells were selected with puromycin. The cells were left unstimulated or were stimulated with human recombinant (hr) IL1b (10 ng/ml) and mouse recombinant (mr) IFNg (10 ng/ml) for six hours and RNA was isolated. Note: Control shRNA, SHC016, is cytotoxic and its expression leads to elimination of shRNA-coding casette (personal observation).
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| Contributor(s) |
Czarnek M, Bereta J |
| Citation(s) |
35107128 |
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| Submission date |
Jul 29, 2021 |
| Last update date |
Mar 15, 2022 |
| Contact name |
Joanna Bereta |
| E-mail(s) |
joanna.bereta@uj.edu.pl
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| Phone |
+48-12-664-6356
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| Organization name |
Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
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| Department |
Cell Biochemistry
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| Street address |
Gronostajowa 7
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| City |
Kraków |
| ZIP/Postal code |
31-387 |
| Country |
Poland |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA750655 |
| SRA |
SRP330415 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE181084_shRNA_RNAseq.xls.gz |
4.8 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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