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| Status |
Public on Oct 01, 2021 |
| Title |
Transcriptomic analysis of A3250 inflammatory breast cancer (IBC) cells, SUM149 IBC and MDA-MB-231 non-IBC cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Transcriptome profiling (RNA-seq) of a novel human Inflammatory Breast Cancer cell line A3250 in comparison to SUM149 and MDA-MB-231
Inflammatory Breast Cancer (IBC) is the most aggressive form of breast cancer with distinct clinical and histopathological features, but understanding of the unique aspects of IBC biology lags far behind that of other breast cancers.
We describe a novel triple-negative IBC cell line, A3250, that recapitulates key features of human IBC in a mouse xenograft model.The purpose of this study was to compare differences in gene expression between A3250 IBC, MDA-MB-231 non-IBC and SUM149 IBC that does not present with typical clinical sympotms of IBC in a mouse model, with the goal of identifying unique molecular features for this unique type of breast cancer
Results: RNA-Seq analysis identified expression profile characteristic for the novel A3250 IBC cell line, compared to SUM149 IBC and MDA-MB-231 non-IBC.
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| Overall design |
RNA-Seq analysis of A3250 IBC tumor cells (n=3), SUM149 IBC tumor cells (n=3) and MDA-MB-231 non-IBC tumor cells (n=3) cultured in vitro.
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| Contributor(s) |
Rogic A, Sun A, Pant I, Aaronson SA, Skobe M |
| Citation(s) |
34824220 |
| |
| Submission date |
Jul 25, 2021 |
| Last update date |
Dec 03, 2021 |
| Contact name |
Mihaela Skobe |
| E-mail(s) |
mihaela.skobe@mssm.edu
|
| Phone |
212-659-5570
|
| Organization name |
The Icahn School of Medicine at Mount Sinai
|
| Department |
Oncological Sciences
|
| Street address |
1425 Madison Ave, Rm 15-20F
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA749567 |
| SRA |
SRP329784 |
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