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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 20, 2021 |
| Title |
Chronic IL-15 stimulation and impaired mTOR signaling and metabolism in natural killer cells during acute myeloid leukemia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse embryonic stem cells, neural progenitor cells and embryonic fibroblasts. We find that lysine 4 and lysine 27 trimethylation effectively discriminates genes that are expressed, poised for expression, or stably repressed, and therefore reflect cell state and lineage potential. Lysine 36 trimethylation marks primary coding and non-coding transcripts, facilitating gene annotation. Trimethylation of lysine 9 and lysine 20 is detected at satellite, telomeric and active long-terminal repeats, and can spread into proximal unique sequences. Lysine 4 and lysine 9 trimethylation marks imprinting control regions. Finally, we show that chromatin state can be read in an allele-specific manner by using single nucleotide polymorphisms. This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations.
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| Overall design |
Examination of 2 different histone modifications in Neural progenitor cells.
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| Contributor(s) |
Bou-Tayeh B, Laletin V, Salem N, Just-Landi S, Fares J, Leblanc R, Balzano M, Kerdiles YM, Bidaut G, Hérault O, Olive D, Aurrand-Lions M, Walzer T, Nunès JA, Fauriat C |
| Citation(s) |
34975835 |
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| Submission date |
Jul 19, 2021 |
| Last update date |
Jan 10, 2022 |
| Contact name |
Richard Tomasini |
| E-mail(s) |
richard.tomasini@inserm.fr
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| Phone |
(+33) 4 91 82 88 15
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| Organization name |
Centre de Recherche en Cancérologie de Marseille
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| Department |
Microenvironment and inter-cellular dialogue
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| Lab |
Pancreatic Cancer Team
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| Street address |
Parc Scientifique de Luminy, 163 avenue de Luminy, case 915
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| City |
Marseille |
| ZIP/Postal code |
13288 |
| Country |
France |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA748074 |
| SRA |
SRP328990 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE180409_CPM_Normalized_Counts.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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