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| Status |
Public on Jul 22, 2021 |
| Title |
The transcriptome-wide N6-methyladenosine (m6A) map profiling reveals the regulatory role of m6A in the yak ovary |
| Organism |
Bos grunniens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The yak estrus is a seasonal phenomenon, probably involving the epigenetic regulation of the synthesis and secretion of sex hormones as well as the growth and development of follicles. In this study, we collected the yak ovarian tissues at the three different states of anestrus, estrus, and pregnancy, and obtained the full transcriptome m6A map in yak by MeRIP-seq. The RT-qPCR showed that the expression of METTL3, METTL14, FTO, YTHDC1 were significantly different across different periods in the ovaries. Next, we identified 20174, 19747 and 13523 m6A peaks in the three ovarian samples of YO-A, YO-F and YO-P using the methylated RNA immunoprecipitation sequencing (MeRIP-seq). The m6A peaks are highly enriched in the coding sequence (CDS) region and 3′untranslated region (3′UTR) as well as the conserved sequence of “RRACH.” Functional analysis revealed the involvement of m6A in many physiological activities of the yak’s ovary during estrus and pregnancy. The association analysis found that some genes such as BNC1, HOMER1, BMP15, BMP6, GPX3, and WNT11 were related to ovarian functions. The comparison of the distribution patterns of methylation peaks in the ovarian tissues across different periods further explored the m6A markers related to the regulation of ovarian ovulation and follicular development in the yak ovary. This comprehensive map provides a solid foundation for revealing the potential function of the mRNA m6A modification in the yak ovary.
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| Overall design |
Detection of m6A in Yak ovaries during anestrus, estrus and pregnancy
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| Contributor(s) |
Guo S, Wang X, Pei J, Guo X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 19, 2021 |
| Last update date |
Jul 22, 2021 |
| Contact name |
Xingdong Wang |
| E-mail(s) |
wxd17339929758@126.com
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| Phone |
17339929758
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| Organization name |
CAAS: Chinese Academy of Agricultural Sciences
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| Street address |
No.335 Jiangouyan, Qilihe District
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| City |
Lanzhou |
| State/province |
未选择 |
| ZIP/Postal code |
730050 |
| Country |
China |
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| Platforms (1) |
| GPL30409 |
Illumina NovaSeq 6000 (Bos grunniens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA748064 |
| SRA |
SRP328976 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE180401_YO_A_IPVSYO_A_input_peak.txt.gz |
2.0 Kb |
(ftp)(http) |
TXT |
| GSE180401_YO_FVSYO_A_diffPeak_result.txt.gz |
8.5 Kb |
(ftp)(http) |
TXT |
| GSE180401_YO_FVSYO_P_diffPeak_result.txt.gz |
49.7 Kb |
(ftp)(http) |
TXT |
| GSE180401_YO_F_IPVSYO_F_input_peak.txt.gz |
3.7 Kb |
(ftp)(http) |
TXT |
| GSE180401_YO_PVSYO_A_diffPeak_result.txt.gz |
1.7 Kb |
(ftp)(http) |
TXT |
| GSE180401_YO_P_IPVSYO_P_input_peak.txt.gz |
37.6 Kb |
(ftp)(http) |
TXT |
| GSE180401_genes_fpkm_expression.txt.gz |
31.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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