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| Status |
Public on Sep 17, 2021 |
| Title |
Epithelial memory of resolved inflammation limits tissue damage while promoting pancreatic tumorigenesis [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Inflammation is a major risk factor for pancreatic ductal adenocarcinoma (PDAC). When occurring in the context of pancreatitis, mutations of KRAS accelerate tumor development. We discovered that long after its complete resolution, a transient inflammatory event primes pancreatic epithelial cells to subsequent transformation by oncogenic KRAS. Upon recovery from acute inflammation, epithelial cells of the pancreas display an enduring adaptive response associated with sustained transcriptional and epigenetic reprogramming. Such adaptation enables the prompt reactivation of acinar-to-ductal metaplasia (ADM) upon subsequent inflammatory events, thus efficiently limiting tissue damage via rapid decrease of zymogen production. We propose that since activating mutations of KRAS maintain an irreversible ADM, they may be beneficial and under strong positive selection in the context of recurrent pancreatitis.
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| Overall design |
Pancreatic spheroids were derived from untreated as well as post caerulein-treated C57BL/6J wild-type mice. Total RNA from pancreatic spheroid was processed for multiparallel sequencing.
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| Contributor(s) |
Del Poggetto E, Ho I, Balestrieri C, Natoli G, Viale A |
| Citation(s) |
34529467 |
| |
| Submission date |
Jul 15, 2021 |
| Last update date |
Sep 20, 2021 |
| Contact name |
Chiara Balestrieri |
| E-mail(s) |
balestrieri.c@gmail.com
|
| Organization name |
IRCCS San Raffaele Scientific Institute
|
| Department |
Center for Omics Sciences
|
| Street address |
Via Olgettina 58
|
| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE180212 |
Epithelial memory of resolved inflammation limits tissue damage while promoting pancreatic tumorigenesis |
|
| Relations |
| BioProject |
PRJNA747027 |
| SRA |
SRP328561 |
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