|
| Status |
Public on Jul 21, 2021 |
| Title |
Sequencing of siRNA from soybean containing silencing vectors and empty vectors |
| Organism |
Glycine max |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
A cDNA library was constructed by Novogene (CA, USA) using a Small RNA Sample Pre Kit, and Illumina sequencing was conducted according to company workflow, using 20 million reads. Raw data were filtered for quality as determined by reads with a quality score > 5, reads containing N < 10%, no 5' primer contaminants, and reads with a 3' primer and insert tag. The 3' primer sequence was trimmed and reads with a poly A/T/G/C were removed
|
| |
|
| Overall design |
Examination of siRNA corresponding to Ss-oah1 in S. sclerotiorum in plants containing silencing constructs and plants containing empty vectors
|
| |
|
| Contributor(s) |
McCaghey M, Shao D, Kurcezewski K, Lindstrom A, Ranjan A, Whitham S, Conley S, Williams B, Smith D, Kabbage M |
| Citation(s) |
34354721 |
| |
| Submission date |
Jul 14, 2021 |
| Last update date |
Aug 11, 2021 |
| Contact name |
Mehdi Kabbage |
| E-mail(s) |
kabbage@wisc.edu
|
| Organization name |
University of Wisconsin Madison
|
| Department |
Department of Plant Pathology
|
| Lab |
583 Russell Labs 1630 Linden Dr.
|
| Street address |
Department of Plant Pathology, 583 Russell Labs 1630 Linden Dr
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL28801 |
Illumina NovaSeq 6000 (Glycine max) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA746614 |
| SRA |
SRP328377 |
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