|
| Status |
Public on Jul 14, 2021 |
| Title |
RNA-seq of glioma and normal cell lines after HDAC1 knock-down |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using RNA-seq we characterized the effect of shRNA mediated knockdown of HDAC1 in glioblastoma stem cells (GSCs), normal ihNPCs, and normal human astrocytes.
|
| |
|
| Overall design |
GSCs, hNPCs and NHAs transduced with either shNT or shHDAC1 shRNAs at a MOI of 3 were selected with 2 ug/mL puromycin 72 hours post-transduction. 72 hours post-selection, total RNA was extracted from cells using the PureLink RNA Mini Kit (Ambion) in accordance to the manufacturer's instructions.
|
| |
|
| Contributor(s) |
Lo Casio C, McNamara J, Melendez EL, Lewis E, Dufault M, Kornblum HI, Sanai N, Plaisier CL, Mehta S |
| Citation(s) |
34494550 |
| |
| Submission date |
Jul 11, 2021 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Christopher L Plaisier |
| E-mail(s) |
plaisier@asu.edu
|
| Phone |
4809656832
|
| Organization name |
Arizona State University
|
| Department |
SBHSE
|
| Street address |
P.O. Box 879709
|
| City |
Tempe |
| State/province |
Arizona |
| ZIP/Postal code |
85287 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (30)
|
|
| Relations |
| BioProject |
PRJNA745459 |
| SRA |
SRP327853 |
Less...
More...