|
| Status |
Public on Aug 04, 2021 |
| Title |
Spatial transcriptomics of human brain metastases |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Spatial transcriptomics of six surgically-resected human brain metastasis samples
|
| |
|
| Overall design |
After surgical resection, parts of six human brain metastases were embedded in OCT and frozen in a dry ice/2-methylbutane bath. 10 μM sections were placed on Visium gene expression slides which were stored at -80C until use. Slides were methanol fixed and stained with H&E and imaged at 10X on a Biotek Lionheart microscope. Tissues were permeabilized and libraries generated according to the manufacturer's protocols. Images for all samples are available in all_spatial_images.tar.gz.
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| |
|
| Contributor(s) |
Hudson WH |
| Citation(s) |
35584630, 35707680 |
| |
| Submission date |
Jul 06, 2021 |
| Last update date |
Jun 27, 2022 |
| Contact name |
William Hudson |
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Cellular Biology
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
| GSM5420749 |
Spatial transcriptomics of a human brain metastasis (patient 15) |
| GSM5420750 |
Spatial transcriptomics of a human brain metastasis (patient 16) |
| GSM5420751 |
Spatial transcriptomics of a human brain metastasis (patient 19) |
| GSM5420752 |
Spatial transcriptomics of a human brain metastasis (patient 24) |
| GSM5420753 |
Spatial transcriptomics of a human brain metastasis (patient 26) |
| GSM5420754 |
Spatial transcriptomics of a human brain metastasis (patient 27) |
|
| Relations |
| BioProject |
PRJNA744217 |
| SRA |
SRP327191 |
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