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| Status |
Public on Jul 08, 2021 |
| Title |
Proteomic analysis of the pathogen surface in cholera identifies host proteins involved in defense and microbiota recognition [I] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The microbial cell surface is a critical site of microbe-host interactions that often control infection outcomes. Defining the set of host proteins present at this interface has been challenging. Here, we used a surface biotinylation approach coupled to quantitative mass spectrometry to identify and quantify both bacterial and host proteins present on the surface of diarrheal fluid-derived V. cholerae in an infant rabbit model of cholera. Our data showed that V. cholerae surfaces were coated with numerous host proteins, whose abundance were driven by the presence of cholera toxin, including the C type lectin SP-D. Mice lacking SP-D had enhanced V. cholerae intestinal colonization. Additional host proteins (AnxA1, LPO and ZAG) capable of binding V. cholerae were also found to recognize distinct taxa of the murine intestinal microbiota, suggesting that these host factors may play roles in intestinal homeostasis in addition to host defense. Proteomic analysis of microbial surfaces is valuable for identifying host interactions that regulate infection and homeostasis with both pathogens and endogenous microbiota alike.
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| Overall design |
We performed RNAseq on proximal and distal small intestine tissues derived from 4 V. cholerae-infected WT and 4 V. cholerae-infected Sftpd KO mice to identify differentially expressed genes during infection.
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| Contributor(s) |
Zoued A, Zhang H, Zhang T, Giorgio RT, Kuehl CJ, Fakoya B, Sit B, Waldor MK |
| Citation(s) |
34675415 |
| |
| Submission date |
Jul 06, 2021 |
| Last update date |
Oct 25, 2021 |
| Contact name |
Matthew K Waldor |
| Organization name |
Harvard Medical School
|
| Department |
Microbiology
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| Lab |
Waldor Lab
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| Street address |
181 Longwood Avenue
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE179530 |
Proteomic analysis of the pathogen surface in cholera identifies host proteins involved in defense and microbiota recognition |
|
| Relations |
| BioProject |
PRJNA744159 |
| SRA |
SRP327150 |
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