NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE179293 Query DataSets for GSE179293
Status Public on Sep 04, 2021
Title equinox RNAi gene expression profiling
Organism Schmidtea mediterranea
Experiment type Expression profiling by high throughput sequencing
Summary Regeneration is a widespread phenomenon that often requires formation of a new tissue outgrowth at wounds called a blastema. Identifying the key signals that trigger blastema formation is therefore fundamental for understanding the mechanistic basis of regeneration. We uncovered a role for the wound epidermis in regeneration initiation in planarians. The gene equinox is expressed within hours of injury in the planarian wound epidermis and encodes a secreted protein conserved in many phyla. Following equinox inhibition, animals failed to form blastemas, reset positional information, and upregulate stem cell (neoblast) proliferation at wounds. Associated with these defects was an inability to maintain wound-induced gene expression. We propose that activation of equinox in the wound epidermis initiates planarian regeneration.
 
Overall design Polyadenylated RNA was isolated from post-pharyngeal fragments at 0, 3, 6, 16, 24, and 48 hours post-amputation (hpa) from planarians following RNAi of a control gene (C.elegans unc-22; 4-5 replicates) and equinox (5 replicates). Worms were fed eight times over four weeks, and cut 7 days after 4th feeding.
 
Contributor(s) Cloutier J, Scimone L, Maybrun C, Reddien P
Citation(s) 35585061
Submission date Jul 01, 2021
Last update date May 27, 2022
Contact name Peter W Reddien
Organization name Whitehead Institute for Biomedical Research
Department MIT Biology
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platforms (1)
GPL15553 Illumina HiSeq 2000 (Schmidtea mediterranea)
Samples (58)
GSM5413785 post-pharyngeal fragments, u220h1
GSM5413786 post-pharyngeal fragments, u220h2
GSM5413787 post-pharyngeal fragments, u220h3
This SubSeries is part of SuperSeries:
GSE179295 equinox is a planarian wound epidermis gene required for the initiation of regeneration
Relations
BioProject PRJNA743077
SRA SRP326538

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE179293_equinox_DEseq_Table.xlsx 9.9 Mb (ftp)(http) XLSX
GSE179293_equinox_reads_collapsed_v6_reorder.txt.gz 2.1 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap