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| Status |
Public on Feb 16, 2022 |
| Title |
Collagen10-Cre;Rosa26-tdTomato bone marrow single cell RNA-sequencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In order to assess the descendants of hypertrophic chondrocytes, we utilized Collagen10-Cre;Rosa26-tdTomato mouse bone marrow harvested at 2 months of age by centrifugation and light Collagenase II digestion. After sequencing and downstream analysis using Seurat, we observed clusters of cells with gene profiles matching classically defined skeletal stem and progenitor cells as well as CXCL12 abundant reticular (CAR) cells. These cells appear to be upstream of both osteoblasts and adipocytes. We conclude that hypertrophic chondrocytes dedifferentiate to this progentior stage before further differentiation.
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| Overall design |
10X Genomics Single cell RNA-sequencing of bone marrow reporter positive cells.
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| Contributor(s) |
Long JT, Leinroth A, Liao Y, Ren Y, Mirando AJ, Nguyen T, Guo W, Sharma D, Wu C, Cheah KS, Karner CM, Hilton MJ, Rouse D |
| Citation(s) |
35179487 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AR063071 |
Notch Signaling in Joint Cartilage Maintenance and Arthritis |
DUKE UNIVERSITY |
Matthew J. Hilton |
| R01 AR071722 |
Notch Signaling in Endochondral Bone Development |
DUKE UNIVERSITY |
Matthew J. Hilton |
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| |
| Submission date |
Jun 29, 2021 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Matthew J. Hilton |
| E-mail(s) |
matthew.hilton@duke.edu
|
| Phone |
9196139761
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| Organization name |
DUKE UNIVERSITY SCHOOL OF MEDICINE
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| Street address |
450 Research Drive, LSRC B321C
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (1) |
| GSM5410631 |
Col10a1-Cre;R26-tdTomato Bone marrow cells post-FACS |
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| Relations |
| BioProject |
PRJNA742416 |
| SRA |
SRP326147 |
