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| Status |
Public on Jun 27, 2021 |
| Title |
Transcriptomic insights of a 1,3-Propanediol producer Clostridium strain isolated from Colombian soil |
| Organism |
Clostridium sp. IBUN13A |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The possibility of establishing a Clostridium-based biorefinery is an attractive and viable alternative, due to the wide metabolic versatility of these microorganisms. The Bioprocesses and Bioprospecting group of the Universidad Nacional de Colombia has obtained the genome of Clostridium sp. IBUN13A, which has shown the ability to produce solvents from various substrates and postulated the need to expand the knowledge of the physiology of the bacteria, so, this study establishes the differences in the transcriptomic profile of the strain when it is cultivated in glycerol respect to glucose after 24 hours of fermentation. For this, a RNA-seq study was carried out, and some of the genes that increased its expression on glycerol were related to the oxidative route of its consumption. The enrichment analysis of Gene Ontology terms showed that the biological phenomena with the highest representation within the differentially expressed genes correspond to oxidation-reduction processes. This first approach to the global transcriptomic study of glycerol fermentation allows the understanding of the metabolism of the microorganism with the purpose of choosing targets for genetic modification.
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| Overall design |
Comparison of expression between glycerol and glucose consumption on a Clostridium butyricum strain
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| Contributor(s) |
Perez-Mancilla X, Riaño-Pachón DM, Montoya-Castaño D |
| Citation missing |
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| |
| Submission date |
Jun 26, 2021 |
| Last update date |
Jun 29, 2021 |
| Contact name |
Ximena Carolina Perez-Mancilla |
| E-mail(s) |
xcperezm@unal.edu.co
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| Organization name |
Universidad Nacional de Colombia
|
| Street address |
Cra 30 #45 - 03
|
| City |
Bogotá D.C. |
| ZIP/Postal code |
111321 |
| Country |
Colombia |
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| Platforms (1) |
| GPL30317 |
Illumina HiSeq 2500 (Clostridium sp. IBUN13A) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA741685 |
| SRA |
SRP325728 |
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