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| Status |
Public on Jun 25, 2021 |
| Title |
Intra-ocular dendritic cells characterize HLA-B27-associated acute anterior uveitis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Uveitis describes a heterogeneous group of inflammatory eye diseases characterized by infiltration of leukocytes into the uveal tissues. uveitis associated with the HLA haplotype B27 (HLA-B27) is a common subtype of uveitis and a prototypical ocular immune-mediated disease. Local immune mechanisms driving human uveitis are poorly characterized mainly due to the limited available biomaterial and subsequent technical limitations. Here, we provide the first high-resolution characterization of intraocular leukocytes in HLA-B27 positive (n=3) and negative (n=2) anterior uveitis and an infectious endophthalmitis control (n=1) by combining single cell RNA-sequencing with flow cytometry and protein analysis. Ocular cell infiltrates consisted primarily of lymphocytes in both subtypes of uveitis and of myeloid cells in infectious endophthalmitis. HLA-B27 positive uveitis exclusively featured a plasmacytoid and classical dendritic cells (cDC) infiltrate. Moreover, cDCs were central in predicted local cell-cell communication. This suggests a unique pattern of ocular leukocyte infiltration in HLA-B27 positive uveitis with relevance of dendritic cells.
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| Overall design |
Single-cell RNA profiling of intraokular leukocytes from HLA-B27 positive (n=3) compared with HLA-B27 negative (n = 2) anterior uveitis and an infectious endophthalmitis (n=1)
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| Contributor(s) |
Kasper M, Heming M, Heiligenhaus A, Meyer zu Hörste G |
| Citation(s) |
34783307 |
| |
| Submission date |
Jun 24, 2021 |
| Last update date |
Nov 21, 2021 |
| Contact name |
Michael Heming |
| Organization name |
University Hospital Muenster
|
| Department |
Department of Neurology with Institute of Translational Neurology
|
| Street address |
Albert-Schweitzer-Campus 1, Bldg A1
|
| City |
Muenster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (7)
|
| GSM5398796 |
single-cell RNA seq data of a patient with HLA-B27neg anterior uveitis , patient#b27neg1 |
| GSM5398797 |
single-cell RNA seq data of a patient with HLA-B27neg anterior uveitis , patient#b27neg2 |
| GSM5398798 |
single-cell RNA seq data of a patient with HLA-B27pos acute anterior uveitis , patient#b27pos1 |
| GSM5398799 |
single-cell RNA seq data of a patient with HLA-B27pos acute anterior uveitis , patient#b27pos2 |
| GSM5398800 |
single-cell RNA seq data of a patient with HLA-B27pos acute anterior uveitis , patient#b27pos3 |
| GSM5398801 |
single-cell RNA seq data of a patient with HLA-B27pos acute anterior uveitis , patient#b27pos4 |
| GSM5398802 |
single-cell RNA seq data of a patient with infectious endophthalmitis , patient#ctrl1 |
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| Relations |
| BioProject |
PRJNA741216 |
| SRA |
SRP325491 |
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