|
| Status |
Public on Sep 01, 2022 |
| Title |
Single cell sequencing of GFP+ Tri-PyMT cells from lung metastasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using an EMT lineage-tracing model, we identified tumor cells in the transitioning phases of both EMT and MET. Further analyses revealed that the ribosome biogenesis (RiBi) pathway was specifically elevated in these transitioning cells, regardless of their destined phenotypical fates.
|
| |
|
| Overall design |
GFP+ Tri-PyMT cells were sorted from parental culture of Tri-PyMT cells by flow cytometry and injected into SCID mice to let lung metastasis outgrowth. 15 days after injection, tumor bearing lungs of 3 mice were harvested and prepare for single cell suspensions. GFP+ cells were sorted for single cell RNA sequencing.
|
| |
|
| Contributor(s) |
Ban Y, Gao D |
| Citation(s) |
39259576 |
| |
| Submission date |
Jun 21, 2021 |
| Last update date |
Oct 08, 2024 |
| Contact name |
Dingcheng Gao |
| E-mail(s) |
dig2009@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Department |
CT Surgery
|
| Street address |
1300 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (1) |
|
| This SubSeries is part of SuperSeries: |
| GSE178579 |
Targeting epithelial-mesenchymal plasticity via ribosome inhibition to reduce chemoresistant metastasis of breast cancer |
|
| Relations |
| BioProject |
PRJNA739682 |
| SRA |
SRP324847 |
