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GEO help: Mouse over screen elements for information. |
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Status |
Public on Dec 02, 2021 |
Title |
Constitutive expression of Progesterone Receptor B in the uterine epithelium prevents implantation by inhibiting estrogen regulation of LIF signaling [RNAseq-PRAoe_PRBoe_oil_P4] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
RNA-seq analysis of uterine luminal epithelium at D4.5 indicates that epithelial PGRA and PGRB shares conseved pathways. Constitutive epithelial PGRA and PGRB disrupts the embryo implantation both through the suppressed FOXO1 signaling by excluding FOXO1 from the nuclear at the uterine epithelium. There are three layers of regulation. Firstly, PGRA and PGRB diminishes Lif transcription in uterine glands by blocking ESR1 binding at the Lif promoter at D3.5 which is critical for the FOXO1 nuclear expression at D4.5 through LIF/pSTAT3/FOXO1. Secondly, PGRA and PGRB directly suppres Foxo1 transcription at the uterine epithelium probably through direct binding at Foxo1 promoter. Thirdly, PGRA and PGRB promotes the Sgk1 transcription, the kinases that phosphorylate FOXO1 to translocate it into cytoplasma for degradation.
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Overall design |
PgrcrePgrALsL/+ and PgrcrePgrBLsL/+ mice were ovariectomized for 2 weeks. And the whole uterus were collected for RNA-Seq after 6h oil or progesterone treatment.
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Contributor(s) |
Li R, Wang T, Zhou L, Wu S, DeMayo FJ |
Citation(s) |
34934913 |
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Submission date |
Jun 21, 2021 |
Last update date |
Jun 30, 2022 |
Contact name |
Franco Demayo |
E-mail(s) |
francesco.demayo@nih.gov
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Organization name |
NIEHS
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Street address |
111 TW Alexander Drive
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City |
RTP |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (16)
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GSM5393561 |
6h oil treated PgrcrePgrALsL/+ mouse uterus, bioreplicate-1 [ePRAoe oil-1] |
GSM5393562 |
6h oil treated PgrcrePgrALsL/+ mouse uterus, bioreplicate-2 [ePRAoe oil-2] |
GSM5393563 |
6h oil treated PgrcrePgrALsL/+ mouse uterus, bioreplicate-3 [ePRAoe oil-3] |
GSM5393564 |
6h oil treated PgrcrePgrALsL/+ mouse uterus, bioreplicate-4 [ePRAoe oil-4] |
GSM5393565 |
6h progesterone treated PgrcrePgrALsL/+ mouse uterus, bioreplicate-1 [ePRAoe P4-1] |
GSM5393566 |
6h progesterone treated PgrcrePgrALsL/+ mouse uterus, bioreplicate-2 [ePRAoe P4-2] |
GSM5393567 |
6h progesterone treated PgrcrePgrALsL/+ mouse uterus, bioreplicate-3 [ePRAoe P4-3] |
GSM5393568 |
6h progesterone treated PgrcrePgrALsL/+ mouse uterus, bioreplicate-4 [ePRAoe P4-4] |
GSM5393569 |
6h oil treated PgrcrePgrBLsL/+ mouse uterus, bioreplicate-1 [ePRBoe oil-1] |
GSM5393570 |
6h oil treated PgrcrePgrBLsL/+ mouse uterus, bioreplicate-2 [ePRBoe oil-2] |
GSM5393571 |
6h oil treated PgrcrePgrBLsL/+ mouse uterus, bioreplicate-3 [ePRBoe oil-3] |
GSM5393572 |
6h oil treated PgrcrePgrBLsL/+ mouse uterus, bioreplicate-4 [ePRBoe oil-4] |
GSM5393573 |
6h progesterone treated PgrcrePgrBLsL/+ mouse uterus, bioreplicate-1 [ePRBoe P4-1] |
GSM5393574 |
6h progesterone treated PgrcrePgrBLsL/+ mouse uterus, bioreplicate-2 [ePRBoe P4-2] |
GSM5393575 |
6h progesterone treated PgrcrePgrBLsL/+ mouse uterus, bioreplicate-3 [ePRBoe P4-3] |
GSM5393576 |
6h progesterone treated PgrcrePgrBLsL/+ mouse uterus, bioreplicate-4 [ePRBoe P4-4] |
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This SubSeries is part of SuperSeries: |
GSE178542 |
The role of epithelial Progesterone Receptor Isoforms in embryo implantation |
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Relations |
BioProject |
PRJNA739581 |
SRA |
SRP324807 |
Supplementary file |
Size |
Download |
File type/resource |
GSE178541_RNAseq-PRAoe_PRBoe_oil_P4-FPKM.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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