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| Status |
Public on Jun 17, 2024 |
| Title |
LncRNA HIF1A-AS1 regulates the apoptosis and proliferation of vascular endothelial cells through globally regulating miRNAs expression [miRNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In the current study, on the basis of establishment of PA-induced damage model of HUVECs, the silencing and overexpression (OE) of HIF1A-AS1 were implemented in the HUVECs, and the effects on cell viability, apoptosis, migration and invasion were explored, respectively. Functionally, the results indicated silencing of HIF1A-AS1 promoted the proliferation migration and invasion, and reduced the apoptosis of HUVECs. In contrast, OE of HIF1A-AS1 reduced proliferation transwell and invasion, promoted the apoptosis, suggesting that HIF1A-AS1 play vital roles in regulating HUVECs. Moreover, miRNA sequencing (miRNA-seq) and RNA sequencing (RNA-seq) were carried out, and the results indicated that HIF1A-AS1 globally mediate expression of miRNA and mRNA. The multiple target genes of DEmiRNA were associated with cell proliferation and apoptosis, and overlapped with the DEGs from RNA-seq, suggestion that HIF1A-AS1 might regulate the proliferation and apoptosis of HUVECs by regulating miRNA expression.
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| |
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| Overall design |
Examination the silencing and overexpression of HIF1A-AS1 in HUVECs
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| |
|
| Contributor(s) |
Chen L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 17, 2021 |
| Last update date |
Jun 17, 2024 |
| Contact name |
Guosong Jiang |
| E-mail(s) |
jiangguosongdoc@hotmail.com
|
| Organization name |
Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
|
| Department |
Department of Urology
|
| Street address |
Jiefang Road, No. 1277
|
| City |
WuHan |
| State/province |
Hubei Province |
| ZIP/Postal code |
430022 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (12)
|
| GSM5389958 |
PA-treated HUVECs-1 [miRNA-Seq] |
| GSM5389959 |
PA-treated HUVECs-2 [miRNA-Seq] |
| GSM5389960 |
PA-treated HUVECs-3 [miRNA-Seq] |
| GSM5389961 |
transfected blank plasmid to PA-treated HUVECs-1 [miRNA-Seq] |
| GSM5389962 |
transfected blank plasmid to PA-treated HUVECs-2 [miRNA-Seq] |
| GSM5389963 |
transfected blank plasmid to PA-treated HUVECs-3 [miRNA-Seq] |
| GSM5389964 |
HIF1A-AS1 overexpression in PA-treated HUVECs-1 [miRNA-Seq] |
| GSM5389965 |
HIF1A-AS1 overexpression in PA-treated HUVECs-2 [miRNA-Seq] |
| GSM5389966 |
HIF1A-AS1 overexpression in PA-treated HUVECs-3 [miRNA-Seq] |
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| This SubSeries is part of SuperSeries: |
| GSE178402 |
LncRNA HIF1A-AS1 regulates the apoptosis and proliferation of vascular endothelial cells through globally regulating miRNAs expression |
|
| Relations |
| BioProject |
PRJNA738875 |
| SRA |
SRP324497 |
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