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| Status |
Public on Jun 15, 2022 |
| Title |
Identification of methylated sites in BT-549 cells affected by thymoquinone treatment |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
BT-549 cells were treated with thymoquinone for 6 h and chromatin immune-precipitation (ChIP) was performed using anti- MBD1 antibody (Abcam, USA). Anti-MBD1 could precipitate specific genomic region, which are methylated. The ChIP products were sequenced by library construction and bioinformatics analysis. The gene ontology (GO) analysis for multiple pathways was performed. The genomic methylated regions affected by TQ treatment were identified.
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| Overall design |
Investigation of thymoquinone's role on genomewide methylation
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| Contributor(s) |
Khan M, Fu J |
| Citation(s) |
35761256 |
| |
| Submission date |
Jun 16, 2021 |
| Last update date |
Sep 17, 2022 |
| Contact name |
Md. Asaduzzaman Khan |
| E-mail(s) |
asadkhanbmj@yahoo.com
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| Organization name |
Southwest Medical University
|
| Department |
The research center for preclinical medicine
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| Lab |
Key laboratory of epigenetics and oncology
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| Street address |
3-319, Zhongshan road
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| City |
Luzhou |
| State/province |
Sichuan |
| ZIP/Postal code |
646000 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA738509 |
| SRA |
SRP324434 |
